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Simultaneous Determination of Lamotrigine and Carbamazepine in Plasma Using Ultrasound-Assisted Emulsification Microextraction-High Performance Liquid Chromatography

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Abstract

A simple, fast and efficient method based on ultrasound-assisted emulsification microextraction (USAEME) followed by HPLC has been developed for simultaneous determination of Carbamazepine (CBZ) and Lamotrigine (LTG) in biological samples. The influence of different parameters affecting the USAEME procedure was evaluated to optimize the efficiency of the process. At these conditions, validation of the proposed method was performed based on European medicines agency guidelines. The proposed method showed good linearity in plasma samples ranging from 0.25 to 6 µg/mL and 0.13 to 6 µg/mL (R2 ≥ 0.99) for CBZ and LTG, respectively. The precision of the proposed method was evaluated for repeatability which was ≤12% (n = 5). The detection limits of 0.06 and 0.05 µg/mL were obtained for CBZ and LTG, respectively. The proposed method can be applied as an effective technique for therapeutic drug monitoring of CBZ and LTG in plasma of epileptic patients.

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Funding

The authors are highly thankful to Department of Chemistry, Boroujerd Branch, Islamic Azad University for providing facilities and all necessary funding to conduct this research.

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Authors and Affiliations

  1. Department of Chemistry, Borujerd Branch, Islamic Azad University, Borujerd, Iran

    Parvin Shahdousti, Mohammad Aghamohammadi, Elahe Fallahi & Hamideh Kalhor

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  1. Parvin Shahdousti
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  2. Mohammad Aghamohammadi
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  3. Elahe Fallahi
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  4. Hamideh Kalhor
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Correspondence to Parvin Shahdousti.

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Parvin Shahdousti, Aghamohammadi, M., Fallahi, E. et al. Simultaneous Determination of Lamotrigine and Carbamazepine in Plasma Using Ultrasound-Assisted Emulsification Microextraction-High Performance Liquid Chromatography. J Anal Chem 75, 622–628 (2020). https://doi.org/10.1134/S1061934820050160

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  • DOI: https://doi.org/10.1134/S1061934820050160

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