Abstract
To characterize of the constituents in rat urine after oral administration of Radix Astragali decoction, a HPLC-DAD-MS/MS technique had been developed. Urine collected from 0 to 24 h, after administration, was purified using a C18 solid-phase extraction cartridge, and then detected by an on-line MS/MS detector. By comparing the retention times and MS/MS data with those obtained from authentic compounds and the published data, a total of 11 compounds including six flavonoids, one dipeptide, one triterpenoid and three of their metabolites in urine were identified. Compounds daidzein, genistein, quercetin, L-asparamide-D-phenylalanine, 4,2′,4′-trihydroxychalcone, Astragaloside-IV, daidzein sulfate, and kaempferol sulfate were for the first time detected by HPLC-MS/MS technique in urine. The present research results success-fully narrowed the range of effective constituents to be found in Astragalus membranaceus and would be useful for the following action mechanism research of this traditional Chinese medicine in treating various diseases.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ye, G., Zhu, H.Y., Li, Z.X., Ma, C.H., Fan, M.S., Sun, Z.L., and Huang C.G., Biomed. Chromatogr., 2007, vol. 21, no. 6, p. 655.
Yang, D.H., Cai, S.Q., Liu, H.Y., Guo, X.X., Li, C.L., Shang, M.Y., Wang, X., and Zhao, Y.Y., J. Chromatogr. B, 2006, vol. 831, nos. 1–2, p. 288.
Ma, C.H., Wang, L.X., Tang, Y.H., Fan, M.S., Xiao, H.B., and Huang, C.G., Biomed. Chromatogr., 2008, vol. 22, no. 10, p. 1066.
Ma, C.H., Li, Z.X., Wang, L.X., Tang, Y.H., Xiao, H.B., and Huang, C.G., Helv. Chim. Acta, 2009, vol. 92, no. 2, p. 379.
Wu, F.B. and Chen, X.Y., Chinese Trad. Herb. Drugs, 2004, vol. 27, no. 3, p. 232.
Wen, Y.M., Chinese Trad. Patent Med., 2006, vol. 28, no. 6, p. 879.
Xu, F., Zhang, Y., Xiao, S.Y., Lu, X.W., Yang, D.H., Yang, X.D., Li, C.L., Shang, M.Y., Tu, P.F., and Cai, S.Q., Drug Metab. Dispos., 2006, vol. 34, no. 6, p. 913.
Han, F.M., Peng, Z.H., Song, W., Zhang, H.M., Zhu, M.M., and Chen, Y., J. Chromatogr. B., 2007, vol. 854, nos. 1–2, p. 1.
Lam, W. and Ramanathan, R., J. Am. Soc. Mass Spectr., 2002, vol. 13, no. 4, p. 345.
Li, L., Tsao, R., Dou, J.P., Song, F.R., Liu, Z.Q., and Liu, S.Y., Anal. Chim. Acta, 2005, vol. 536, nos. 1–2, p. 21.
Zhao, F.Z. and Zhuang, B.N., J. Nanjing Med. Univ., 1995, vol. 15, p. 270.
Li, R.F., Zhou, Y.Z., Qiao, L., Fu, H.W., and Pei, Y.H., J. Shenyang Pharm. Univ., 2007, vol. 24, no. 1, p. 20.
Xiao, H.B., Krucker, M., Putzbach, K., and Albert, K., J. Chromatogr. A, 2005, vol. 1067, nos. 1–2, p. 135.
Heinonen, S.M., Hoikkala, A., Wähälä, K., and Adlercreutz, H., J. Steroid Biochem. Mol. Biol., 2003, vol. 87, nos. 4–5, p. 285.
Dian, L.H., Wu, T., and Lu, Y.N., Mod. Med. Health, 2007, vol. 23 p. 2550.
March, R.E., Lewars, E.G., Stadey, C.J., Miao, X.S., Zhao, X.M., and Metcalfe, C.D., Int. J. Mass Spectrom., 2006, vol. 248, nos. 1–2, p. 61.
Author information
Authors and Affiliations
Additional information
The article is published in the original.
Rights and permissions
About this article
Cite this article
Zhang, Z.Y., Wu, B., Tang, Y.H. et al. Characterization of major flavonoids, triterpenoid, dipeptide and their metabolites in rat urine after oral administration of Radix Astragali decoction. J Anal Chem 68, 716–721 (2013). https://doi.org/10.1134/S1061934813080145
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934813080145