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Haplotype variation of estrogen receptor-α (ER-α) gene exon 4 in Turkish sheep breeds

  • Animal Genetics
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Abstract

Estrogen receptor a (ERα) gene has previously been found related with sexual development and reproduction. In this study, on the basis of the sequences of human, cattle and caprine estrogen receptor α (ERα) genes, available in the GenBank database, sets of PCR primers were designed and used to amplify the ovine ERα gene exon 4 region. We identified six single nucleotide polymorphisms (SNP) in the ERα exon 4. Some variations determined for exon 4 g.43A > G, p.T43A; g.49C > T, p.L49F; g.178A > T, p.T178S led to changes in the amino acids, but no amino acid changes were determined in g.18G > C, g.27C > T, g.96G > A. These fragments were deposited in the GenBank database under accession numbers: JF262030-JF262035. It was noted in particular that White Karaman and Awassi breeds were similar to each other, whereas the Chios breed had a different variation.

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Authors and Affiliations

  1. Faculty of Veterinary, Medicine Department of Genetic, Firat University, Elazig, Turkey

    O. Ozmen

  2. Faculty of Veterinary, Medicine Department of Animal Breeding, Firat University, Elazig, Turkey

    I. Seker

  3. Faculty of Veterinary, Medicine Department of Genetic, Ankara University, Ankara, Turkey

    B. Cinar Kul & O. Ertugrul

Authors
  1. O. Ozmen
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  2. I. Seker
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  3. B. Cinar Kul
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  4. O. Ertugrul
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Correspondence to O. Ozmen.

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Ozmen, O., Seker, I., Cinar Kul, B. et al. Haplotype variation of estrogen receptor-α (ER-α) gene exon 4 in Turkish sheep breeds. Russ J Genet 48, 1015–1019 (2012). https://doi.org/10.1134/S1022795412100067

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  • DOI: https://doi.org/10.1134/S1022795412100067

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