Skip to main content
SpringerLink
Log in

The in vivo genotoxic effects of sodium metabisulfite in bone marrow cells of rats

  • Animal Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

This study is designed to investigate the genotoxic effect of sodium metabisulphite (SMB), which is used as an antimicrobial substance in foods on bone marrow cells of rats. Four different concentrations of SMB (250, 500, 750 and 1000 mg/kg body weight) were given rats (Rattus norvegicus var. albinos) for 6, 12 and 24 h treatment period by intraperitoneal (IP) and gavage (GV) administrations. In this study, we found that intraperitoneal implement of SMB generally more effectively increases the percentage of abnormal cells and CA/cell in all concentrations and treatment period. In addition, mitotic index (MI) data of intraperitoneal injection are lower than gavage. It can be concluded that potential genotoxic effects of SMB by IP injection are higher than GV injection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Abe, S. and Sasaki, M., Chromosome Aberrations and Sister Chromatid Exchanges in Chinese Hamster Cells Exposed to Various Chemicals, J. Natl. Cancer Inst., 1977, vol. 58, pp. 1635–1643.

    PubMed  CAS  Google Scholar 

  2. Nagao, M., Honda, M., Seino, Y., et al., Mutagenicities of Protein Pyrolates, Cancer Lett., 1977, vol. 2, pp. 335–339.

    Article  PubMed  CAS  Google Scholar 

  3. Ishidate, Jr., M. and Odashima, S., Chromosome Test with 134 Compounds on Chinese Hamster Cells in vitro-a Screening for Chemical Carcinogens, Mutat. Res., 1977, vol. 48, pp. 337–353.

    PubMed  CAS  Google Scholar 

  4. Matsuoka, A., Hayashi, M., and Ishidate, Jr.M., Chromosomal Aberration Test on 29 Chemical Combined with S9 Nix in vitro, Mutat. Res., 1979, vol. 66, pp. 277–290.

    Article  PubMed  CAS  Google Scholar 

  5. Njagi, G.D. and Gopalan, H.N., Cytogenetic Effects of the Food Preservatives-Sodium Benzoate and Sodium Sulphite on Vicia faba Root Meristems, Mutat. Res., 1982, vol. 102, pp. 213–219.

    Article  PubMed  CAS  Google Scholar 

  6. Ishidate, Jr., M., Sofuni, T., Yoshikawa, K., et al., Primary Mutagenicity Screening of Food Additives Currently Used in Japan, Food Chem. Toxicol., 1984, vol. 22, pp. 623–636.

    Article  PubMed  CAS  Google Scholar 

  7. Hasegawa, M.M., Nishi, Y., Ohkawa, Y., and Inui, N., Effects of Sorbic Acid and Its Salts on Chromosome Aberrations, Sister Chromatid Exchanges and Gene Mutations in Cultured Chinese Hamster Cells, Food Chem. Toxicol., 1984, vol. 22, pp. 501–507.

    Article  PubMed  CAS  Google Scholar 

  8. Luca, D., Luca, V., Cotor, F., and Raileanu, L., In vivo and in vitro Cytogenetic Damage Induced by Sodium Nitrite, Mutat. Res., 1987, vol. 189, pp. 333–339.

    Article  PubMed  CAS  Google Scholar 

  9. Pagano, D.A. and Zeiger, E., Conditions Affecting the Mutagenicity of Sodium Bisulfite in Salmonella typhimurium, Mutat. Res., 1987, vol. 2, pp. 159–166.

    Google Scholar 

  10. De Giovanni-Donnelly, R., The Mutagenicity of Sodium Bisulfite on Bas-Substitution Strains of Salmonella typhimurium, Teratog. Carcinog. Mutagen., 1985, vol. 3, pp. 195–203.

    Article  Google Scholar 

  11. Mukherje, A., Giri, A.K., Talukder, G., and Sharma, A., Sister Chromatid Exchanges and Micronuclei Formations Induced by Sorbic Acid and Sorbic Acid Nitrite in vivo in Mice, Toxicol. Lett., 1988, vol. 42, pp. 47–53.

    Article  Google Scholar 

  12. Munzer, R., Guigas, C., and Renner, H.W., Re-Examination of Potassium Sorbate and Sodium Sorbate for Possible Genotoxic Potential, Food Chem. Toxicol., 1990, vol. 28, pp. 397–401.

    Article  Google Scholar 

  13. Akin, A. and Sümer, S., The Mutagenic Effects of Sodium Nitrite and Monosodium Glutamate Used as Food Additives Demonstrated by the Salmonella microsome Test System, Microbiol. Bull., 1991, vol. 25, pp. 94–107.

    CAS  Google Scholar 

  14. Rencuzoğullari, E., Kayraldiz, A., İla, H.B., et al., The Cytogenetic Effects of Sodium Metabisulphite, a Food Preservative in Root Tip Cells of Allium cepa L., Turk. J. Biol., 2001, vol. 25, pp. 361–370.

    Google Scholar 

  15. Rencuzoğullari, E., İla, H.B., Kayraldiz, A., and Topaktas, M., Chromosome Aberrations and Sister Chromatid Exchanges in Cultured Human Lymphocytes Treated with Sodium Metabisulphite, Mutat. Res., 2001, vol. 490, pp. 107–112.

    PubMed  Google Scholar 

  16. Lavoie, J.C. and Chessex, P., Biologic Effects of Parenteral Bisulfite on Human Vascular Tissue, Pediatr. Res., 1993, vol. 33, pp. 347–351.

    Article  PubMed  CAS  Google Scholar 

  17. Sun, J., Sakamoto, T., and Chung, K.F., Effects of Sodium Metabisulphite on Guinea Pig Contractile Airway Smooth Muscle Responses in vitro, Mutat. Res., 2003, vol. 471, pp. 112–119.

    Google Scholar 

  18. The Ministry of Agricultural of Turkey Food Codex Instructions, Turkey: Globus World Publ., 1997.

  19. Meng, Z.Q. and Zhang, L., Chromosomal Aberrations and Sister Chromatid Exchanges in Lymphocytes of Workers Exposed to Sulphur Dioxide, Mutat. Res., 1990, vol. 241, pp. 15–20.

    Article  PubMed  CAS  Google Scholar 

  20. Hayatsu, H., Bisulfite Modification of Nucleic Acids and Their Constituents, Progr. Nucl. Acid Res. USA, 1976, vol. 58, pp. 543–547.

    Google Scholar 

  21. Shapiro, R., Genetic Effects of Bisulfite, Mutat. Res., 1977, vol. 39, pp. 149–154.

    PubMed  CAS  Google Scholar 

  22. Munzer, R., Untersuchungen zur Mutagen Wirkung von Bisulfit, Lebensm. Weiss., 1980, vol. 29, pp. 1871–1874.

    Google Scholar 

  23. MacRae, W.D. and Stich, H.F., Induction of Sister Chromatid Exchanges in Chinese Hamster Cells by the Reducing Agents Bisulphite and Ascorbic Acid, Toxicology, 1979, vol. 13, pp. 167–171.

    PubMed  CAS  Google Scholar 

  24. Meng, Z. and Zhang, L., Cytogenetic Damage Induced in Human Lymphocytes by Sodium Bisulfite, Mutat. Res., 1992, vol. 298, pp. 63–69.

    Article  PubMed  CAS  Google Scholar 

  25. Meng, Z. and Zhang, B., Polymerase Chain Reaction-Based Deletion Screening of Bisulfite (Sulphur Dioxide)-Enhanced GPT-Mutants in CHO-AS52 Cells, Mutat. Res., 1999, vol. 425, pp. 81–85.

    PubMed  CAS  Google Scholar 

  26. Mace, M.L., Jr., Daskal, Y., and Wray, W., Scanning Electron Microscopy of Chromosome Aberrations, Mutat. Res, 1978, vol. 52, pp. 199–206.

    PubMed  Google Scholar 

  27. Bhanot, O.S., Aoyagi, S., and Chambers, R.W., Bisulfite Induced C Changed to U Transitions in Yeast Valine tRNA, J. Biol. Chem., 1977, vol. 252, pp. 2566–2574.

    PubMed  CAS  Google Scholar 

  28. Bhanot, O.S. and Chambers, R.W., Bisulfite Induced C Changed to U Transitions in Yeast Alanine tRNA, J. Biol. Chem., 1977, vol. 252, pp. 2551–2559.

    PubMed  CAS  Google Scholar 

  29. Simmons, R.R. and Friedberg, E.C., Enzymatic Degradation of Uracil-Containing Deoxyribonucleic Acid: V. Survival of Escherichia coli and Coliphages Treated with Sodium Bisulfite, J. Bacteriol., 1979, vol. 137, pp. 1243–1252.

    PubMed  CAS  Google Scholar 

  30. Chen, H. and Shaw, B.R., Bisulfite Induces Tandem Double CC → TT Mutations in Double Strand DNA: 2. Kinetics of Cytosine Deamination, Biochemistry, 1994, vol. 33, pp. 4121–4129.

    Article  PubMed  CAS  Google Scholar 

  31. Hayatsu, H. and Shiragami, M., Reaction of Bisulfite with the 5-Hydroxymethyl Group in Pyrimidines and Phage DNAs, Biochemistry, 1979, vol. 18,, pp. 632–637.

    Article  PubMed  CAS  Google Scholar 

  32. Ashby, J. and Ishidate, M., Jr., Clastogenicity in vitro of Na, K, Ca and Mg Salts of Saccharin, and of Magnesium Chloride: Consideration of Significance, Mutat. Res., 1986, vol. 163, pp. 63–73.

    PubMed  CAS  Google Scholar 

  33. Nowak, C., Studies on the Ability of Hypotonic Solutions to Induce Chromosomal Aberrations in V79 Cells, Teratog. Carcinog. Mutagen., 1987, vol. 7, pp. 515–525.

    Article  PubMed  CAS  Google Scholar 

  34. Nowak, C., Induction of Chromosomal Aberrations by Hypotonic Culture Conditions is Independent of the SPhase in V79 Hamster Cells, Environ. Mol. Mutagen., 1989, vol. 13, pp. 44–49.

    Article  PubMed  CAS  Google Scholar 

  35. Renner, H.W. and Wever, J., Attempts to Induce Cytogenetic Effects with Sulphite Oxidase-Deficient Chinese Hamster and Mice, Food Chem. Toxicol., 1983, vol. 21, pp. 123–127.

    Article  PubMed  CAS  Google Scholar 

  36. Mavournin, K.H., Blakey, D.H., Cimino, M.C., et al., The in vivo Micronucleus Assay in Mammalian Bone Marrow and Peripheral Blood, a Report of the US Environmental Protection Agency Gene-Tox Program, Mutat. Res., 1990, vol. 239, pp. 29–80.

    PubMed  CAS  Google Scholar 

  37. Perry, P.E. and Thomson, E.J., The Methodology of Sister Chromatid Exchanges, Handbook of Mutagenicity Test Procedures, Kilbey, B.J., Legator, M., Nicholas, W., and Ramel, C., Eds., Amsterdam: Elsevier, 1984, 2nd ed., pp. 459–529.

    Google Scholar 

  38. Preston, R.J., Dean, B.J., Galloway, S., et al., Mammalian in vivo Cytogenetic Assay, Analysis of Chromosome Aberrations in Bone Marrow Cells, Mutat. Res., 1987, vol. 189, pp. 157–165.

    Article  PubMed  CAS  Google Scholar 

  39. Meng, Z. and Zhang, B., Induction Effects of Sulfur Dioxide Inhalation on Chromosomal Aberrations in Mouse Bone Marrow Cells, Zhonghua Yu Fang Yi Xue Za Zhi, 2002, vol. 36, pp. 229–231.

    PubMed  CAS  Google Scholar 

  40. Yi, H. and Meng, Z., Genotoxicty of Hyrated Sulfur Dioxide on Root Tips of Allium sativum and Vicia faba, Mutat. Res., 2003, vol. 537, pp. 109–114.

    PubMed  CAS  Google Scholar 

  41. Nakajima, M., Kitazawa, M., Oba, K., et al., Effect of Route of Administration in the Micronucleus Tests with Potassium Bromate, Mutat. Res., 1989, vol. 223, pp. 399–402.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biology Department, Faculty of Arts and Sciences, University of Kahramanmaraş Sütçü Imam, 4210, Kahramanmaraş, Turkey

    A. Kayraldiz

  2. Biology Department, Faculty of Arts and Sciences, University of Çukurova, 01330, Adana, Turkey

    M. Topaktaş

Authors
  1. A. Kayraldiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Topaktaş
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Kayraldiz.

Additional information

The text was submitted by the authors in English.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kayraldiz, A., Topaktaş, M. The in vivo genotoxic effects of sodium metabisulfite in bone marrow cells of rats. Russ J Genet 43, 905–909 (2007). https://doi.org/10.1134/S1022795407080121

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795407080121

Keywords

Search

Navigation