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Functional Genomic Analysis of the SPL9 Gene in Arabidopsis thaliana under Low Phosphate Conditions

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Abstract

Inorganic phosphate (Pi) is an essential nutrient, which is often served as a limiting factor in plant growth. It has been reported that SPL family members, such as SPL3, regulate Pi deficiency responses by controlling the expression of Pi deficiency responsive genes. To elucidate whether SPL9 respond to low phosphorus stress, we investigated the phenotypes and conducted RNA sequencing analysis in transgenic Arabidopsis thaliana with overexpressing rSPL9 (R9) under conditions of both normal and low Pi availability. Compared with wild-type plants, R9 showed decreased anthocyanin accumulation and increased Pi contents in shoots under Pi deficiency. Through RNA-seq analysis compared with wild-type plants, we detected 217 genes significantly differentially expressed in conditions of Pi sufficiency, and 121 genes differentially expressed in conditions of Pi deficiency in R9 plants. Under Pi deficiency, MYB62 and ZAT6 are two important differentially expressed genes (DEGs) that both regulate the Pi uptake processes. In addition, these DEGs included multiple protein kinases, jasmonic acid response genes and genes related to salt stress responses. Genes associated with hydrolase and transferase activity were also differentially regulated by Pi deficiency, such as cytochrome P450 monooxygenases. Of particular note, the transcription factor AP2-EREBP and members of the bHLH family were among the most significantly differentially regulated genes identified under both Pi sufficient and Pi deficient conditions. In conclusion, our analysis of the R9 transcriptome highlights the importance of SPL9 under conditions of Pi-deficiency. Except for stress and defense response genes, the R9 transcriptome also characterized the induction of ethylene or jasmonic acid signaling under Pi deficiency.

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ACKNOWLEDGMENTS

We thank Professor Jiawei Wang for providing the overexpressing rSPL9 seeds.

Funding

This work was supported by the National Natural Science Foundation of China (grant nos. 31601140 and 31900241).

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Authors and Affiliations

  1. Pharmacy Institute of Henan University, 475004, Kaifeng, China

    K.-J. Lei

  2. Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China

    H. Dong

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  1. K.-J. Lei
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  2. H. Dong
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Correspondence to H. Dong.

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Abbreviations: R9—overexpression of rSPL9.

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Lei, KJ., Dong, H. Functional Genomic Analysis of the SPL9 Gene in Arabidopsis thaliana under Low Phosphate Conditions. Russ J Plant Physiol 69, 32 (2022). https://doi.org/10.1134/S1021443722020091

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