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SPINK3: A novel growth factor that promotes rat liver regeneration

  • Molecular Cell Biology
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Abstract

Serine peptidase inhibitor, Kazal type 3 (SPINK3) is a trypsin inhibitor, and also a growth factor that has an identical structure to epidermal growth factor (EGF), which could combine with epidermal growth factor receptor (EGFR) to promote cell proliferation. To shed light on the role and regulation mechanism of SPINK3 in rat liver regeneration (LR), Rat Genome 230 2.0 assay was used to detect the expression profiles of LR genes after partial hepatectomy (PH). The results showed that Spink3 was significantly up-regulated at 2–24 h and 72–168 h after PH. In the present study, RT-PCR and immunoblotting were used to validate the assay results. Ingenuity Pathway Analysis 9.0 (IPA) software was used to build the SPINK3 signaling regulating LR and analyze the possible mechanism. And then the expression of cell proliferation-associated gene Ccna2 was examined by RT-PCR in normal rat liver cell line BRL-3A in which Spink3 was overexpressed. The results showed that Ccna2 was significantly up-regulated in BRL-3A in which Spink3 was over-expressed. SPINK3 combining with EGFR accelerated cell proliferation during rat liver regeneration via P38, PKC, JAK-STAT and AKT pathways. Thus, SPINK3 was likely to promote hepatocytes proliferation in LR through P38, PKC, JAK-STAT and AKT pathways.

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Abbreviations

BCG:

blank control group

EG:

experimental group

LR:

liver regeneration

NC:

normal control

NCG:

negative control group

PH:

partial hepatectomy

SO:

sham-operated (rats)

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Authors and Affiliations

  1. State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, 453007, P.R. China

    C. F. Chang, J. Yang, X. F. Li, W. M. Zhao, S. S. Chen, G. P. Wang & C. S. Xu

  2. College of Life Science, Henan Normal University, Xinxiang, 453007, P.R. China

    C. F. Chang, J. Yang, X. F. Li, W. M. Zhao, S. S. Chen, G. P. Wang & C. S. Xu

Authors
  1. C. F. Chang
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  2. J. Yang
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  3. X. F. Li
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  4. W. M. Zhao
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  5. S. S. Chen
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  6. G. P. Wang
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  7. C. S. Xu
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Correspondence to C. S. Xu.

Additional information

Published in Russian in Molekulyarnaya Biologiya, 2016, Vol. 50, No. 3, pp. 457–465.

The article is published in the original.

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Chang, C.F., Yang, J., Li, X.F. et al. SPINK3: A novel growth factor that promotes rat liver regeneration. Mol Biol 50, 398–404 (2016). https://doi.org/10.1134/S0026893316030055

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  • DOI: https://doi.org/10.1134/S0026893316030055

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