Abstract
Recent studies have suggested that contactin-1 has a key role in cancer cell proliferation and migration, however the detailed mechanism of this process is still unclear. Here, human gastric cancer cell line MKN45 was employed. It was found that under hypoxia conditions contactin-1 mRNA and protein levels were both up-regulated by HIF-1α expression. Furthermore, although hypoxia increased the migration rate of MKN45 cells, contactin-1 (CNTN1) shRNA reversed this process. Meanwhile, RhoA V14 and RhoA V14 N19 mutation constructs were employed, and it was found that constitutively active form of RhoA reversed the cell migration suppression induced by contactin-1 knockdown, while dominant-negative form of RhoA blocked hypoxia induced hypermigration. Apart from this, contactin-1 displayed the ability to phosphorylate the RhoA activator p115 RhoGEF. Thus, under hypoxia conditions, elevated HIF-1α seems to up-regulate contactin-1 expression and by this activate RhoA and facilitate migration of cancer cells.
Similar content being viewed by others
References
Fidler I.J. 2003. The pathogenesis of cancer metastasis: The “seed and soil” hypothesis revisited. Nat. Rev. Cancer. 3, 453–458.
Han F.H., Li H.M., Zheng D.H., He Y.L., Zhan W.H. 2010. The effect of the expression of vascular endothelial growth factor (VEGF)-C and VEGF receptor-3 on the clinical outcome in patients with gastric carcinoma. Eur. J. Surg. Oncol. 36, 1172–1179.
Dicken B.J., Bigam D.L., Cass C., Mackey J.R., Joy A.A., Hamilton S.M. 2005. Gastric adenocarcinoma review and considerations for future directions. Ann. Surg. 241, 27–39.
Monteiro P., Rossé C., Castro-Castro A., Irondelle M., Lagoutte E., Paul-Gilloteaux P., Desnos C., Formstecher E., Darchen F., Perrais D., Gautreau A., Hertzog M., Chavrier P. 2013. Endosomal WASH and exocyst complexes control exocytosis of MT1-MMP at invadopodia. J. Cell Biol. 203, 1063–1079.
Sun M., Zhou T., Zhou L., Chen Q., Yu Y., Yang H., Zhong K., Zhang X., Xu F., Cai S., Yu A., Zhang H., Xiao R., Xiao D., Chui D. 2012. Formononetin protects neurons against hypoxia-induced cytotoxicity through upregulation of ADAM10 and sAβPPα. J. Alzheimers Dis. 28,795–808.
Lippitz B.E. 2013. Cytokine patterns in patients with cancer: a systematic review. Lancet Oncol. 14, e218–228.
Fackler O.T., Grosse R. 2008. Cell motility through plasma membrane blebbing. J. Cell Biol. 181, 879–884.
Reid R.A., Bronson D.D., Young K.M., Hemperly J.J. 1994. Identification and characterization of the human cell adhesion molecule contactin. Brain Res. Mol. Brain Res. 21, 1–8.
Liu Y., Ye Z., Luo H., Sun M., Li M., Fan D., Chui D. 2009. Inhalative formaldehyde exposure enhances aggressive behavior and disturbs monoamines in frontal cortex synaptosome of male rats. Neurosci. Lett. 464, 113–116.
Su J.L., Yang C.Y., Shih J.Y., Wei L.H., Hsieh C.Y., Jeng Y.M., Wang M.Y., Yang P.C., Kuo M.L. 2006. Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma. Cancer Res. 66, 2553–2561.
Yu J.W., Wu S.H., Lu R.Q., Wu J.G., Ni X.C., Zhou G.C., Jiang H.G., Zheng L.H., Li X.Q., Du G.Y., Jiang B.J. 2013. Expression and significances of contactin-1 in human gastric cancer. Gastroenterol. Res. Pract. 2013, 210205–210215.
Yan J., Wong N., Hung C., Chen W.X., Tang D. 2013. Contactin-1 reduces E-cadherin expression via activating AKT in lung cancer. PLoS ONE. 8, e65463.
Chang C.C., Shih J.Y., Jeng Y.M., Su J.L., Lin B.Z., Chen S.T., Chau Y.P., Yang P.C., Kuo M.L. 2004. Connective tissue growth factor and its role in lung adenocarcinoma invasion and metastasis. J. Natl. Cancer Inst. 96, 364–375.
Sullivan R., Graham C.H. 2007. Hypoxia-driven selection of the metastatic phenotype. Cancer Metastasis Rev. 26, 319–331.
Fujiwara S., Nakagawa K., Harada H., Nagato S., Furukawa K., Teraoka M., Seno T., Oka K., Iwata S., Ohnishi T. 2007. Silencing hypoxia-inducible factor-1alpha inhibits cell migration and invasion under hypoxic environment in malignant gliomas. Int. J. Oncol. 30, 793–802.
Matsuyama T., Nakanishi K., Hayashi T., Yoshizumi Y., Aiko S., Sugiura Y., Tanimoto T., Uenoyama M., Ozeki Y., Maehara T. 2005. Expression of hypoxia-inducible factor-1alpha in esophageal squamous cell carcinoma. Cancer Sci. 96, 176–182.
Lee J.J., Natsuizaka M., Ohashi S., Wong G.S., Takaoka M., Michaylira C.Z., Budo D., Tobias J.W., Kanai M., Shirakawa Y., Naomoto Y., Klein-Szanto A.J., Haase V.H., Nakagawa H. 2010. Hypoxia activates the cyclooxygenase-2-prostaglandin E synthase axis. Carcinogenesis. 31, 427–434.
Park S.T., Kim B.R., Park S.H., Lee J.H., Lee E.J., Lee S.H. 2013. Suppression of VEGF expression through interruption of the HIF-1α and Akt signaling cascade modulates the anti-angiogenic activity of DAPK in ovarian carcinoma cells. Oncol. Rep. 31, 1021–1029.
Xu H., Li B., Yu W., Wang H., Zhao X., Yao Y. 2013. Correlation between F-FDG uptake and the expression of glucose transporter-1 and hypoxia-inducible factor-1α in transplanted VX2 tumors. Nucl. Med. Commun. 34, 953–958.
Çlakoğlu G., Bergstrom-Tyrberg U., Berglund E.O., Ranscht B. 2014. Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system. Proc. Natl. Acad. Sci. U. S. A. 111, E394-403.
Lamprianou S., Chatzopoulou E., Thomas J.L., Bouyain S., Harroch S. 2011. A complex between contactin-1 and the protein tyrosine phosphatase PTPRZ controls the development of oligodendrocyte precursor cells. Proc. Natl. Acad. Sci. U. S. A. 108, 17498–17503.
Su J.L., Yang P.C., Shih J.Y., Yang C.Y., Wei L.H., Hsieh C.Y., Chou C.H., Jeng Y.M., Wang M.Y., Chang K.J., Hung M.C., Kuo M.L. 2006. The VEGF-C/Flt-4 axis promotes invasion and metastasis of cancer cells. Cancer Cell. 9, 209–223.
Zhang A., Wang Q., Han Z., Hu W., Xi L., Gao Q., Wang S., Zhou J., Xu G., Meng L., Chen G., Ma D. 2013. Reduced expression of Snail decreases breast cancer cell motility by downregulating the expression and inhibiting the activity of RhoA GTPase. Oncol. Lett. 6, 339–346.
Hanna S., Khalil B., Nasrallah A., Saykali B.A., Sobh R., Nasser S., El-Sibai M. 2014. StarD13 is a tumor suppressor in breast cancer that regulates cell motility and invasion. Int. J. Oncol. 44, 1499–1511.
Tong J.J., Yan Z., Jian R., Tao H., Hui O.T., Jian C. 2012. RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia. Oncol. Lett. 4, 495–500.
Yu Y., Zhou L., Sun M., Zhou T., Zhong K., Wang H., Liu Y., Liu X., Xiao R., Ge J., Tu P., Fan D.S., Lan Y., Hui C., Chui D. 2012. Xylocoside G reduces amyloid-β induced neurotoxicity by inhibiting NF-κB signaling pathway in neuronal cells. J. Alzheimers Dis. 30, 263–275.
Iden S., Collard J.G. 2008. Crosstalk between small GTPases and polarity proteins in cell polarization. Nat. Rev. Mol. Cell Biol. 9, 846–859.
Birukova A.A., Birukov K.G., Smurova K., Adyshev D., Kaibuchi K., Alieva I., Garcia J.G., Verin A.D. 2004. Novel role of microtubules in thrombin-induced endothelial barrier dysfunction. FASEB J. 18, 1879–1890.
Chen Z., Guo L., Hadas J., Gutowski S., Sprang S.R., Sternweis P.C. 2012. Activation of p115-RhoGEF requires direct association of Gα13 and the Dbl homology domain. J. Biol. Chem. 287, 25490–25500.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Russian in Molekulyarnaya Biologiya, 2015, Vol. 49, No. 1, pp. 129–137.
The article is published in the original.
The authors contributed equally to the work.
Rights and permissions
About this article
Cite this article
Yang, G., Song, J.G., Li, Y. et al. Under hypoxia conditions contactin-1 regulates the migration of Mkn45 cells through the RhoA pathway. Mol Biol 49, 112–119 (2015). https://doi.org/10.1134/S0026893315010185
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0026893315010185