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Dairy biofilm: Bacterial community diversity assessment and impact of the Lactococcus lactis bio adhesion on biofilm growth

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Abstract

Biofilms are the most common mode of bacterial growth in nature. Their formation occurs on organic or inorganic solid surfaces in contact with a liquid, on gas-liquid and liquid-liquid boundaries as well. The aims of this study were, by combining cell enumeration, scanning electron microscopy and denaturing gel gradient electrophoresis (DGGE), to characterize the structural dynamics of dairy biofilm growth in the environments with a nutrient flow, and to evaluate the impact of adhesion of Lactococcus lactis on the biofilm community depending on the incubation time. Significantly higher values of biofilm volume and thickness were observed under dynamic conditions after 55 h. The populations of gram-positive bacteria and fungi exhibited a significantly higher biofilm organization after 2 days of cultivation than that of gram-negative bacteria. Also, results showed that Lc. lactis was able to adhere to silicone surface and the produced biofilm in which the number of adhered gram-positive and gram-negative bacteria decreased by nine orders of magnitude after 48 h of contact. This study constitutes a step ahead in developing the strategies to prevent microbial colonization by lactococcal protective biofilm.

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Authors and Affiliations

  1. Laboratoire d’Ecologie et de Technologie Microbienne, INSAT/ESIAT, Tunis, Tunisie

    H. Ksontini, F. Kachouri & M. Hamdi

  2. Laboratoire Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Campus Universitaire, Tunis, 2092, Tunisie

    A. Guesmi & A. Cherif

Authors
  1. H. Ksontini
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  2. F. Kachouri
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  3. A. Guesmi
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  4. A. Cherif
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  5. M. Hamdi
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Correspondence to M. Hamdi.

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Ksontini, H., Kachouri, F., Guesmi, A. et al. Dairy biofilm: Bacterial community diversity assessment and impact of the Lactococcus lactis bio adhesion on biofilm growth. Microbiology 82, 364–372 (2013). https://doi.org/10.1134/S002626171303017X

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