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SDS-PAGE for Myosin Heavy Chains: Fast and Furious

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Abstract

Using our own new modification of one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) we successfully separated myosin heavy chains from different chambers of rat heart. The key features of modification are changes in the content and buffer composition of the separating gel, and employment of constant current instead of voltage. These changes allow carrying out a safer and faster one-dimensional SDS-PAGE for cardiac myosin heavy chains using significantly fewer reagents.

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Funding

This work was implemented using the equipment of the Shared Research Center of Scientific Equipment of Institute of Immunology and Physiology within the Governmental assignment to the Institute of Immunology and Physiology of the Russian Academy of Sciences; theme reg. no. 122022200089-4.

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Authors and Affiliations

  1. Institute of Immunology and Physiology of Russian Academy of Sciences, Yekaterinburg, Russia

    O. P. Gerzen, Iu. K. Potoskueva & L. V. Nikitina

  2. Institute of Chemical Engineering of Ural Federal University, Yekaterinburg, Russia

    Yu. V. Permyakova, A. V. Grebenschchikova & I. S. Selezneva

Authors
  1. O. P. Gerzen
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  2. Iu. K. Potoskueva
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  3. Yu. V. Permyakova
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  4. A. V. Grebenschchikova
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  5. I. S. Selezneva
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  6. L. V. Nikitina
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Contributions

Idea and design of the experiment: L.V.N. and O.P.G.; data collection and processing: O.P.G., I.K.P., Y.V.P, and S.R.N.; manuscript writing and editing: O.P.G. and L.V.N.

Corresponding author

Correspondence to O. P. Gerzen.

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CONFLICT OF INTEREST

The authors declare that they have no conflicts of interest.

Additional information

Translated by A. Polyanovsky

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Gerzen, O.P., Potoskueva, I.K., Permyakova, Y.V. et al. SDS-PAGE for Myosin Heavy Chains: Fast and Furious. J Evol Biochem Phys 58 (Suppl 1), S92–S97 (2022). https://doi.org/10.1134/S0022093022070109

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  • DOI: https://doi.org/10.1134/S0022093022070109

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