Abstract
Effect of prednisolone on isolated preparations of trachea of normal rats and rats with fibrosing alveolitis was studied. Prednisolone at a concentration of 0.4 μg/l decreased responses of smooth muscle on stimulation of preganglionic nerve fibers at trachea areas with intramural ganglia in rats with acute alveolitis by 48%, while in normal rats—by 19% of control. In trachea preparations without ganglia, prednisolone at a dose of 10 μg/l decreased responses of muscle to the nerve fiber stimulation by 21.3%. The higher prednisolone doses were less efficient: 0.1–10 μg/l glucocorticoid practically did not affect the smooth muscle responses produced by stimulation of muscle cells. In rats with fibrosing alveolitis, 10 μg/l prednisolone restored the smooth muscle responses to control values in preparations of trachea with intramural ganglia. After the prednisolone treatment, amplitude of the rat trachea muscle contraction in response to the nerve fiber electric stimulation did not differ statistically significantly from control and 0.1–10 μg/l prednisolone did not change the response value. The conclusion is made that prednisolone affected the diseased rats more efficiently than the healthy animals. The character of the glucocorticoid effect depends on the presence of intramural ganglia in the trachea wall.
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References
Barnes, P.J., Pathophysiology of Asthma, Br. Clin. Pharmacol., 1996, vol. 42, pp. 3–10.
Douglas, W.W., Ryu, J.H., and Schroeder, D.R., Idiopathic Pulmonary Fibrosis: Impact of Oxygen and Colchicines? Prednisone? Or no Therapy on Survival, Amer. J. Respir. Crit. Care Med., 2000, vol. 159, pp. 1172–1178.
Interstitsial’nye zabolevaniya legkikh (Interstitial Lung Diseases), Ilkovich, M.M. and Kokosova, A.N., Eds., St. Petersburg, 2005.
Emala, C.W., Clancy, J., and Hirshman, C.A., Glucocorticoid Treatment Decreases Muscarinic Receptor Expression in Canine Airway Smooth Muscle, Am. J. Physiol., 1997, vol. 272(4 Pt 1), pp. L745–L751.
Mak, J.C., Nishikawa, M., Shirasaki, H., et al., Protective Effects of Glucocorticoid on Downregulation of Pulmonary Beta 2-Adrenergic Receptors in vivo, J. Clin. Invest., 1995, vol. 96, no. 1, pp. 99–106.
Florio, C., Flessar, M., Martin, J.G., and Heisler, S., Identification of Adenylate Cyclasecoupled Histamine H2 Receptors in Guinea Pig Smooth Muscle Cells in Culture and Effect of Dexametasone, Am. J. Respir Cell. Mol. Biol., 1992, vol. 7, pp. 582–589.
Hardy, E., Farahani, M., and Hall, I.P., Regulation of Histamine H1 Receptor Coupling by Dexametasone in Human Cultured Airway Smooth Muscle, Br. J. Pharmacol., 1996, vol. 118, pp. 1079–1084.
Danilov, L.N., Lebedeva, E.S., Makhovenko, L.V., et al., Pathogenetic Peculiarities of Formation of Experimental Lung Fibrosis, Aktual’nye problemy pul’monologii (Actual Problems of Pulmonology), Leningrad, 1991, pp. 141–143.
De Jongste, J.C., Jongejan, R.C., and Kerrebijn, K.F., Control of Airway Caliber by Autonomic Nerves in Asthma and in Chronic Obstructive Pulmonary Disease, Am. Rev. Respir. Dis., 1991, vol. 143, pp. 1421–1426.
Ichinose, M., Airway Autonomic Nervous System Dysfunction and Asthma, Nippon Yakurigaku Zasshi, 1998, vol. 111, no. 4, pp. 195–203.
Burns, G.P. and Gibson, J.G., Airway Hyperresponsiveness in Asthma, Am. J. Respir. Crit. Care Med., 1998. vol. 158, no. 1, pp. 203–206.
Marquardt, D.L., Motulsky, H.J., and Wasserman, S.I., Rat Lung Cholinergic Receptor: Characterization and Regulation by Corticosteroids, J. Appl. Physiol., 1982, vol. 53, pp. 731–736.
Agarwal, M.K., The Antiglucocorticoid Action of Mifepristone, Pharmacol. Ther., 1996, vol. 70, pp. 183–213.
Fedin, A.N., Nur, M.M., Kryukova, E.N., and Vasilieva, K.A., Effect of Dexametasone on Rat Airways, Zh. Evol. Biokhim. Fiziol., 2001, vol. 37, pp. 131–135.
Vachnier, I., Chiappara, G., Vignola, A.M., Chigliardo, R., Altieri, E., Terouanne, B., Vic, P., Bousquet, J., Gadard, P., and Chanez, P., Glucocorticoid Receptors in Bronchial Epithelial Cells in Asthma, Am. J. Crit. Care Med., 1998, vol. 158, pp. 963–970.
Fedin, A.N., Kryukova, E.N., and Nekrasova, E.A., Interaction of Histamine and Glucocorticoids with Airway Nerve Structures, Ross. Fiziol. Zh., 2001, vol. 87, pp. 1413–1421.
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Original Russian Text © A. N. Fedin, E. A. Nekrasova, S. A. Frolova, L. N. Danilov, E. S. Lebedeva, and M. M. Il’kovich, 2007, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2007, Vol. 43, No. 4, pp. 352–356.
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Fedin, A.N., Nekrasova, E.A., Frolova, S.A. et al. Effect of prednisolone on trachea smooth muscle of normal rats and rats with fibrosing alveolitis. J Evol Biochem Phys 43, 421–426 (2007). https://doi.org/10.1134/S0022093007040072
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DOI: https://doi.org/10.1134/S0022093007040072