Skip to main content
SpringerLink
Log in

Control Parameters for Separation and Concentration of Rat Erythrocytes and Lymphocytes and Human Erythrocytes in the Field of a Standing Ultrasonic Wave

  • CELL BIOPHYSICS
  • Published:
Biophysics Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

The parameters of controlling the separation of erythrocyte and lymphocyte cells were studied; the possibility of rapid (minutes) separation of blood cells into fractions in the field of a standing ultrasound wave was shown. Seven main control parameters were identified; they were related to the energy of the field of the standing ultrasonic wave, the linear pumping rate of the cell suspension, the geometric dimensions of the cells, the ratio of cell densities and suspending media, and the ratio of ultrasound velocities in the cell material and suspending media. It was shown that under effective temperature control of the chambers of the ultrasonic separation and concentration systems with a suspension of cells, it is possible to significantly (by more than an order of magnitude) reduce the time of separation and concentration of cells not only of different types, but also of similarly-sized cells of the same type isolated from the blood of different animal species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.

Similar content being viewed by others

REFERENCES

  1. N. S. Bardell, J. Sound Vib. 174, 655 (1994).

  2. J. J. Hawkes and W. T. Coakley, Sens. Actuators, B 75, 213 (2001).

    Article  Google Scholar 

  3. C. M. Cousins, J. R. Melin, W. A. Venables, and W. T. Coakley, Bioseparation 9, 343 (2001).

    Article  Google Scholar 

  4. R. Bosma, W. A. van Spronsen, J. Tramper, and R. H. Wijffels, J. Appl. Phycol. 15, 143 (2003).

    Article  Google Scholar 

  5. G. D. Pangu and D. L. Feke, Chem. Eng. Sci. 64, 1445 (2009).

    Article  Google Scholar 

  6. T. Franke, S. Braunmuller, L. Schmid, et al., Lab-on-a-Chip 10, 789 (2010).

    Article  Google Scholar 

  7. T. Franke, A. R. Abate, D. A. Weitza, and A. Wixforth, Lab-on-a-Chip 18, 2625 (2009).

    Article  Google Scholar 

  8. D. Foresti, N. Bjelobrk, M. Nabavi, and D. Poulikakos, J. Appl. Phys. 109, 093503 (2011). https://doi.org/10.1063/1.3571996

    Article  ADS  Google Scholar 

  9. G. R. Goddard, C. K. Sanders, J. C. Martin, et al., Anal. Chem. 79, 8740 (2007).

    Article  Google Scholar 

  10. N. N. Knyaz’kov and G. V. Shil’nikov, Bull. Exp. Biol. Med. 121, 287 (1996).

    Article  Google Scholar 

  11. N. N. Knyaz’kov, E. D. Makarova, and A. D. Rabizhanovich, Sci. Instrum. 19, 40 (2008).

    Google Scholar 

  12. V. A. Shutilov, Basic Physics of Ultrasonic (Leningrad. Gos. Univ., Leningrad, 1980), pp. 114–117.

    Google Scholar 

  13. L. B. Kheifets and V. A. Abalakina, Lab. Delo, No. 10, 579 (1973).

    Google Scholar 

  14. J. C. Fiala, in Proc. Int. Joint Conf. on Neural Networks (Honolulu, HI, 2002), pp. 1–4.

    Google Scholar 

  15. T. N. Pashovkin and D. G. Sadikova, Acoust. Phys. 55 (4–5), 584 (2009).

Download references

Author information

Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    T. N. Pashovkin & D. G. Sadikova

Authors
  1. T. N. Pashovkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. G. Sadikova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. N. Pashovkin.

Ethics declarations

Conflict of interest. The authors declare that they have no conflicts of interest.

All applicable international, national and institutional principles for the care and use of animals in the performance of work have been observed. The procedures performed in the study with the participation of people corresponded to the ethical standards of the Helsinki Declaration of 1964 and its subsequent amendments. Informed voluntary consent to participate in the study was previously obtained from all participants.

Additional information

Translated by E. Puchkov

Abbreviations: LUS, longitudinal ultrasonic selection.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pashovkin, T.N., Sadikova, D.G. Control Parameters for Separation and Concentration of Rat Erythrocytes and Lymphocytes and Human Erythrocytes in the Field of a Standing Ultrasonic Wave. BIOPHYSICS 67, 445–450 (2022). https://doi.org/10.1134/S0006350922030174

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006350922030174

Keywords:

Search

Navigation