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The Use of ToF-SIMS for Analysis of Bioorganic Samples

  • Cell Biophysics
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Abstract

The technique for the preparation of biological tissue sections developed for Electron Probe Microanalysis has been adapted for ToF-SIMS analysis of mouse GV stage oocytes. GV-oocyte sample preparation involves the following steps: plunge freezing, freeze drying, impregnation in an embedding medium, and section cutting. Molecule-specific images of the distribution of molecules in a single oocyte have been obtained with the described technique and ToF-SIMS analysis. The ToF-SIMS analysis data show that the efficient lateral image resolution is approximately 1 μm. Hence, ToF-SIMS enables us to study the distribution of chemical substances in relation to the morphological data obtained by scanning electron microscopy or conventional light microscopy.

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Abbreviations

ToF-SIMS:

Time-of-Flight Secondary Ion Mass Spectroscopy

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    A. G. Pogorelov, V. N. Pogorelova, A. I. Panait & M. A. Pogorelova

  2. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, 119991, Russia

    A. A. Gulin & V. A. Nadtochenko

  3. Department of Chemistry, Moscow State University, Moscow, 119234, Russia

    A. A. Gulin & V. A. Nadtochenko

  4. Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow oblast, 142432, Russia

    V. A. Nadtochenko

Authors
  1. A. G. Pogorelov
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  2. A. A. Gulin
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  3. V. N. Pogorelova
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  4. A. I. Panait
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  5. M. A. Pogorelova
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  6. V. A. Nadtochenko
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Correspondence to A. G. Pogorelov.

Additional information

Original Russian Text © A.G. Pogorelov, A.A. Gulin, V.N. Pogorelova, A.I. Panait, M.A. Pogorelova, V.A. Nadtochenko, 2018, published in Biofizika, 2018, Vol. 63, No. 2, pp. 303–310.

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Pogorelov, A.G., Gulin, A.A., Pogorelova, V.N. et al. The Use of ToF-SIMS for Analysis of Bioorganic Samples. BIOPHYSICS 63, 215–221 (2018). https://doi.org/10.1134/S0006350918020197

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  • DOI: https://doi.org/10.1134/S0006350918020197

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