Abstract
Boron neutron-capture therapy of cancer is based on the ability of the 10B isotope to capture thermal neutrons; it is one of the most promising techniques in radiation therapy. The high content and selective accumulation of 10B in the tumor tissue are the most important prerequisites for its efficacy. The purpose of this study was to determine the biodistribution of cobalt bis(dicarbollide) conjugates with chlorin e 6 amino amide derivatives. Experiments were carried out in Balb/c mice with transplanted CT-26 murine colon carcinoma. Boron-containing conjugates were injected into the tail vein at a dose of 10 mg/kg. The conjugate accumulation in tumor tissue and organs was studied by laser scanning microscopy. Excitation was performed at the wavelength of 514 nm; the signals were recorded in the range of 560–710 nm in increments of 10 nm. To evaluate the amount of the boron conjugate, we calculated the intensity of the fluorescence signal of the samples under investigation. At 3 h after administration of the agent, a high level of fluorescence was observed in the liver, spleen, and lung. The tumor/muscle accumulation ratio was approximately 3. The study demonstrated that boron derivatives of chlorin e 6 are promising agents for boron neutron-capture therapy.
Similar content being viewed by others
Abbreviations
- BNCT:
-
boron neutron-capture therapy
References
I. B. Sivaev, V. I. Bregadze, and N. T Kuznetsov, Izv. Ross. Akad. Nauk, Ser. Khim. No. 8, 1256 (2002).
V. D. Rozymenko, Ukr. Neirokhirurg. Zh. 3, 4 (2001).
W. A. G. Sauerwein, A. Wittig, R. Moss, and Y. Nakagawa, Neutron Capture Therapy: Principles and Applications (Springer, Berlin, 2012).
N. M. Goudgaon, G. F. El-Kattan, and R. F. Schinazi, Nucleosides Nucleotides 13, 849 (1994).
T. Sano, in Advances in Neutron Capture Therapy, Vol. 2: Chemistry and Biology, Ed. by B. Larsson, J. Crawford, and R. Weinreich (Elsevier, Amsterdam, 1997), p.408.
Y. Yamamoto, J. Cai, H. Nakamura, et al., J. Org. Chem. 60, 3352 (1995).
V. A. Brattsev, J. H. Morris, and G. H. Danilova, in Boron Chemistry at the Beginning on the 21st Century, Ed. by Yu. Bubnov (URSS, Moscow, 2003), p.321.
D. A. Buzatu, J. G. Wilkes, D. Miller, et al., US Patent 7 824 660 (September 13, 2011).
H. Yang, L. Jing, Y. Bando, et al., J. Mater. Chem. 20 (5), 1007 (2010).
Yu. N. Bubnov, Boron Chemistry at the Beginning of the 21st Century (URSS, Moscow, 2003).
A. H. Soloway, W. Tjarks, B. A. Barnum, et al., Chem. Rev. 98 (4), 1515 (1998).
I. A. Brattsev and J. H. Morris, in Advances in Neutron Capture Therapy, Vol. 2: Chemistry and Biology, Ed. by B. Larsson, J. Crawford, and R. Weinreich (Elsevier, Amsterdam, 1997), p.51.
A. V. Feofanov, I. A. Lobanova, I. B. Sivaev, et al., Izv. Ross. Akad. Nauk, Ser. Khim. No. 1, 215 (2010).
A. V. Efremenko, A. A. Ignatova, M. A. Grin, et al., Photochem. Photobiol. Sci. 13 (1), 92 (2014).
A. V. Efremenko, A. A. Ignatova, M. A. Grin, et al., in Current Microscopy Contributions to Advances in Science and Technology, Ed. by A. Mendez-Vilas (FORMATEX, Spain, 2012), pp. 84–90.
V. A. Ol’shevskaya, A. V. Zaytsev, A. N. Savchenko, et al., Bull. Korean Chem. Soc. 28 (11), 1910 (2007).
M. A. Grin, R. A. Titeev, D. I. Brittal, et al., Mendeleev Commun. 21, 84 (2011).
S. S. Kuznetsov, L. B. Snopova, M. M. Karabut, et al., Sovr. Tekhnol. Med. 7 (3), 32 (2015).
R. I. Freshney, Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 5th ed. (Wiley, Hoboken, NJ, 2005; BINOM, Moscow, 2010).
A. Juzeniene, Photodiagn. Photodynamic Ther. 6 (2), 94 (2009).
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.B. Volovetskiy, N.Y. Shilyagina, V.V. Dudenkova, S.O. Pasynkova, A.A. Ignatova, A.F. Mironov, M.A. Grin, V.I. Bregadze, A.V. Feofanov, I.V. Balalaeva, A.V. Maslennikova, 2016, published in Biofizika, 2016, Vol. 61, No. 1, pp. 158–164.
Rights and permissions
About this article
Cite this article
Volovetskiy, A.B., Shilyagina, N.Y., Dudenkova, V.V. et al. Study of the tissue distribution of potential boron neutron-capture therapy agents based on conjugates of chlorin e 6 aminoamide derivatives with boron nanoparticles. BIOPHYSICS 61, 133–138 (2016). https://doi.org/10.1134/S0006350916010255
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006350916010255