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Investigation of membrane permeability of carp spermatozoa for water molecules

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Abstract

The fundamentals of a photometry method for determination of membrane permeability of some fish spermatozoa for water molecules are presented. Osmotic tolerance of carp spermatozoa membranes was studied using EPR-spectroscopy and photometric analysis methods. It was shown that carp spermatozoa look like the ideal osmometers in their reaction on media of different osmolarity. The value of membrane permeability of carp spermatozoa for water molecules was determined. Data obtained can be used in cryobiology for creating cryoprotective media and regimes of fish sperm cryopreservation.

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Authors and Affiliations

  1. Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, 61015, Ukraine

    A. Yu. Pugovkin, E. F. Kopeika, O. A. Nardid & Ya. O. Cherkashina

Authors
  1. A. Yu. Pugovkin
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  2. E. F. Kopeika
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  3. O. A. Nardid
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  4. Ya. O. Cherkashina
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Correspondence to A. Yu. Pugovkin.

Additional information

Original Russian Text © A.Yu. Pugovkin, E.F. Kopeika, O.A. Nardid, Ya.O. Cherkashina, 2014, published in Biofizika, 2014, Vol. 59, No. 3, pp. 481–487.

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Pugovkin, A.Y., Kopeika, E.F., Nardid, O.A. et al. Investigation of membrane permeability of carp spermatozoa for water molecules. BIOPHYSICS 59, 393–398 (2014). https://doi.org/10.1134/S000635091403018X

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  • DOI: https://doi.org/10.1134/S000635091403018X

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