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Analysis of mitochondrial DNA somatic mutations in OXYS and Wistar strain rats

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Abstract

Rats of the OXYS strain are sensitive to oxidative stress and serve as a biological model of premature aging. We have compared spectra of somatic mutations in a control region of mtDNA from the liver of the OXYS rat strain and of Wistar rats as a control. The majority of nucleotide substitutions in the mutation spectra were represented by transitions: 94 and 97% in the OXYS and Wistar rats, respectively. It was shown that 40% of somatic mutations in the control region of mtDNA from Wistar rats were significantly consistent with the model of dislocation mutagenesis. No statistical support for this model was found for mutations in the control region of mtDNA from OXYS rats. The mutation frequency in the ETAS section was higher in the OXYS strain rats than in Wistar rats. These results suggest different mechanisms of mutagenesis in the two rat strains under study.

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Abbreviations

mtDNA:

mitochondrial DNA

8-oxoG:

8-oxoguanine

Pol γ:

DNA polymerase γ

ROS:

reactive oxygen species

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Author information

Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, pr. Lavrent’eva 10, 630090, Novosibirsk, Russia

    U. N. Rotskaya, I. B. Rogozin, E. A. Vasyunina, N. G. Kolosova, G. A. Nevinsky & O. I. Sinitsyna

  2. Institute of Biological Problems of the North, Far-Eastern Division of the Russian Academy of Sciences, ul. Portovaya 18, 685000, Magadan, Russia

    B. A. Malyarchuk

  3. Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, pr. Lavrent’eva 8, 630090, Novosibirsk, Russia

    G. A. Nevinsky

Authors
  1. U. N. Rotskaya
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  2. I. B. Rogozin
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  3. E. A. Vasyunina
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  4. N. G. Kolosova
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  5. B. A. Malyarchuk
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  6. G. A. Nevinsky
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  7. O. I. Sinitsyna
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Correspondence to O. I. Sinitsyna.

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Original Russian Text © U. N. Rotskaya, I. B. Rogozin, E. A. Vasyunina, N. G. Kolosova, B. A. Malyarchuk, G. A. Nevinsky, O. I. Sinitsyna, 2009, published in Biokhimiya, 2009, Vol. 74, No. 4, pp. 532–541.

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Rotskaya, U.N., Rogozin, I.B., Vasyunina, E.A. et al. Analysis of mitochondrial DNA somatic mutations in OXYS and Wistar strain rats. Biochemistry Moscow 74, 430–437 (2009). https://doi.org/10.1134/S0006297909040117

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  • DOI: https://doi.org/10.1134/S0006297909040117

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