Skip to main content
SpringerLink
Log in

Enzymatic transformation of biologically active 1,3;1,6-β-D-glucan. Structure and activity of resulting fragments

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

The fragmentation of the biologically active 1,3;1,6-β-D-glucan Antivir by endo-1,3-β-D-glucanase LIV from crystalline styles of the marine mollusk Spisula sachalinensis was carried out. It was found that low molecular mass oligomers possessing a stabilizing effect on membranes and anti-viral activity against tobacco mosaic virus appeared in the process of enzymatic hydrolysis of Antivir. Biological activity of 1,3;1,6-β-D-glucooligo-and polysaccharides was found to be associated with molecular mass (polymerization degree (n) not less than 14) and with presence of intralinked β-1,6-connected monosaccharide residues. Probably, decrease in molecular mass is compensated by increase in number of intralinked β-1,6-connected monosaccharide residues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bohn, J. A., and BeMiller, J. N. (1995) Carbohydr. Polym., 28, 3–14.

    Article  CAS  Google Scholar 

  2. Albersheim, P., and Darvill, A. G. (1985) Sci. Am., 253, 58–64.

    Article  Google Scholar 

  3. Kopp, M., Rouster, J., Fritig, B., Darvill, A., and Albersheim, P. (1989) Plant Physiol., 90, 208–216.

    Article  PubMed  CAS  Google Scholar 

  4. Albersheim, P., Darvill, A., Augur, C., Cheong, J. J., Eberhard, S., Hahn, M. G., Marfa, V., Mohnen, D., O’Neill, M. A., Spiro, M. D., and York, W. S. (1992) Acc. Chem. Res., 25, 77–83.

    Article  CAS  Google Scholar 

  5. Rouhier, P., Kopp, M., Begot, V., Bruneteau, M., and Fritig, B. (1995) Phytochemistry, 39, 57–62.

    Article  PubMed  CAS  Google Scholar 

  6. Varum, K. M., Kvam, B. J., and Paulsen, S. M. (1986) Carbohydr. Res., 152, 243–248.

    Article  Google Scholar 

  7. Dalmo, R. A., Bogwald, J., Ingebrigtsen, K., and Seljelid, R. (1996) J. Fish Diseases, 19, 449–457.

    Article  CAS  Google Scholar 

  8. Zvyagintseva, T. N., Elyakova, L. A., and Isakov, V. V. (1995) Bioorg. Khim., 21, 218–225.

    CAS  Google Scholar 

  9. Chertkov, K. S., Davydova, S. A., Nesterova, T. A., Zvyagintseva, T. N., and Elyakova, L. A. (1999) Radiats. Biol. Radioekol., 39, 572–577.

    CAS  Google Scholar 

  10. Reunov, A. V., Lapshina, L. A., Nagorskaya, V. P., and Elyakova, L. A. (1996) J. Phytopathol., 144, 247–249.

    CAS  Google Scholar 

  11. Elyakova, L. A., Likhatskaya, G. N., Aminin, D. L., and Zvyagintseva, T. N. (1995) Russian Federation Patent No. 2034026.

  12. Zvyagintseva, T. N., Besednova, N. N., and Elyakova, L. A. (2002) Structure and Immunotropic Effects of 1,3; 1,6-β-D-Glucans [in Russian], Dalnauka, Vladivostok, p. 157.

    Google Scholar 

  13. Sova, V. V., and Elyakova, L. A. (1972) Biochim. Biophys. Acta, 258, 219–227.

    PubMed  CAS  Google Scholar 

  14. Privalova, N. M., and Elyakova, L. A. (1978) Comp. Biochem. Physiol., 60B, 225–228.

    CAS  Google Scholar 

  15. Zvyagintseva, T. N., Shevchenko, N. M., Popivnich, I. B., Isakov, V. V., Scobun, A. S., Sundukova, E. V., and Elyakova, L. A. (1999) Carbohydr. Res., 322, 32–39.

    Article  CAS  Google Scholar 

  16. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956) Analyt. Chem., 28, 350–356.

    Article  CAS  Google Scholar 

  17. Nelson, N. (1944) J. Chem., 153, 375–381.

    CAS  Google Scholar 

  18. Wistler, R. (ed.) (1964) Methods in Carbohydrate Chemistry, Academic Press, New York-London.

    Google Scholar 

  19. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) J. Biol. Chem., 193, 265–275.

    PubMed  CAS  Google Scholar 

  20. Otsuki, Y., Takebe, I., Onho, T., Fukuda, M., and Okada, Y. (1977) Proc. Natl. Acad. Sci. USA, 74, 1913–1917.

    Article  PubMed  CAS  Google Scholar 

  21. (1988) Methods for Cultivation of Animal Cells [in Russian], Nauka, Leningrad.

  22. Bezukladnikov, P. W., Elyakova, L. A. (1990) Carbohydr. Res., 203, 119–127.

    Article  CAS  Google Scholar 

  23. Sova, V. V., Zvyagintseva, T. N., Svetasheva, T. G., Burtseva, Yu. V., and Elyakova L. A. (1997) Biochemistry (Moscow), 62, 1113–1118.

    CAS  Google Scholar 

  24. Likhatskaya, G. N., Elyakova, L. A., Aminin, D. L., Agafonova, I. G., and Zvyagintseva, T. N. (1997) Abstr. II Congr. of Russian Biochemical Society, Moscow, Pt. II, p. 332.

  25. Shevchenko, N. M., Zvyagintseva, T. N., and Elyakova, L. A. (1986) Carbohydr. Res., 148, 57–62.

    Article  CAS  Google Scholar 

  26. Elyakova, L. A., and Dmitrenok, P. (1991) Bioorg. Khim., 17, 860–863.

    CAS  Google Scholar 

  27. Elyakova, L. A., and Nazarova, N. I. (1995) Bioorg. Khim., 21, 55–60.

    CAS  Google Scholar 

  28. Elyakova, L. A., Bakunina, I. Yu., Mamontova, V. A., and Isakov, V. V. (1998) Bioorg. Khim., 24, 211–218.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, 690022, Vladivostok, Russia

    L. A. Elyakova, V. V. Isakov, L. A. Lapshina, V. P. Nagorskaya, G. N. Likhatskaya, T. N. Zvyagintseva & A. V. Reunov

Authors
  1. L. A. Elyakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. V. Isakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. A. Lapshina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. P. Nagorskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. N. Likhatskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. N. Zvyagintseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. V. Reunov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. N. Zvyagintseva.

Additional information

Original Russian Text © L. A. Elyakova, V. V. Isakov, L. A. Lapshina, V. P. Nagorskaya, G. N. Likhatskaya, T. N. Zvyagintseva, A. V. Reunov, 2007, published in Biokhimiya, 2007, Vol. 72, No. 1, pp. 36–44.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM06-004, December 10, 2006.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Elyakova, L.A., Isakov, V.V., Lapshina, L.A. et al. Enzymatic transformation of biologically active 1,3;1,6-β-D-glucan. Structure and activity of resulting fragments. Biochemistry (Moscow) 72, 29–36 (2007). https://doi.org/10.1134/S0006297907010038

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006297907010038

Key words

Search

Navigation