Abstract
In the course of bioassay-guided study for natural antidiabetic compounds from microorganisms, two steroids, i.e. compounds 1 and 2, were isolated in vitro from biomass of antidiabetic Streptomyces sp. strain TD-X10. Structures of the compounds 1 and 2 were elucidated on the basis of spectroscopic analysis of nuclear magnetic resonance and mass spectral data and comparison with reported literature data as β-sitosterol and β-sitosteryl ferulate, respectively. While compound 1 exhibited faintly inhibitory activity against protein tyrosine phosphatase 1B, a highly validated therapeutic target for diabetes mellitus, compound 2 displayed a moderate inhibitory effect to the enzyme with IC50 value of 40.05 ± 2.46 µM. The results highlight the antidiabetic potential of natural products from actinobacteria, particularly Streptomyces species.
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Funding
This research work was financially supported by Ministry of Agriculture and Rural Development (Project “Study on production and application of organophosphorus (OP) degrading microbial bioproducts for bioremediation and crop yield enhancement”) and Vietnamese Academy of Science and Technology (VAST) [grant of VAST 0205/17-18].
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Hoang, C.K., Nguyen, V.T., Tran, H.T. et al. Isolation and Structure Determination of PTP1B Inhibitor from Streptomyces sp. Strain TD-X10. Appl Biochem Microbiol 57, 65–73 (2021). https://doi.org/10.1134/S0003683821010099
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DOI: https://doi.org/10.1134/S0003683821010099