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Study of the Accumulation of Rec A from Bacillus subtilis in the Mitochondria of a Recombinant Strain of the Yeast Yarovia lipolytica

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Abstract

No eukaryotic species has a system for homologous DNA recombination of the mitochondrial genome. We report on an integrative genetic system based on the pQ-SRUS construct that allows the expression of the RecA recombinase from Bacillus subtilis and its transportation to mitochondria of Yarrowia lipolytica. The targeting of recombinant RecA to mitochondria is provided by leader sequences (5'-UTR and 3'-UTR) derived from the SOD2 gene mRNA, which exhibit affinity to the outer mitochondrial membrane and provides cotranslational import of RecA to the inner space of mitochondria. The accumulation of RecA in mitochondria of the Y. lipolytica recombinant strain bearing the pQ-SRUS construct has been shown by immunoblotting of purified mitochondrial preparations.

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References

  1. Di Mauro, S., Bonilla, E., Zeviani, M., Nakagawa, M., and DeVivo, D.C., Ann. Neurol., 1985, vol. 17, no. 6, pp. 521–538.

    Article  CAS  Google Scholar 

  2. Sukernik, R.I., Derbeneva, O.A., Starikovskaya, E.B., Volod’ko, N.V., Mikhailovskaya, I.E., Bychkov, I.Yu., Lott, M.T., Braun, M.D., and Uolles, D.K., Genetika, 2002, vol. 38, no. 2, pp. 1–10.

    Google Scholar 

  3. Di Mauro, S., Biochim Biophys Acta, 2004, vol. 1659, nos. 2–3, pp. 107–114.

    Article  CAS  Google Scholar 

  4. Di Mauro, S. and Schon, E.A., Annu. Rev. Neurosci., 2008, vol. 31, pp. 91–123.

    Article  CAS  Google Scholar 

  5. Di Donato, S., J. Neurol., 2009, vol. 256, pp. 693–710.

    Article  PubMed  Google Scholar 

  6. Naviaux, R.K., Mitochondrion, 2004, vol. 4, nos 5–6, pp. 351–361.

    Article  CAS  PubMed  Google Scholar 

  7. Anderson, S., Bankier, A.T., Barrell, B.G., et al., Nature, 1981, vol. 290, no. 5806, pp. 457–465.

    Article  CAS  PubMed  Google Scholar 

  8. Kmiec, B., Woloszynska, M., and Janska, H., Curr. Genet., 2006, vol. 50, no. 3, pp. 149–59.

    Article  CAS  PubMed  Google Scholar 

  9. Wonnapinij, P., Chinnery, P.F., and Samuels, D.C., Am. J. Hum. Genet., 2008, vol. 83, no. 5, pp. 582–593.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Krasnopol'skaya, K.D. and Zakharova, E.Yu., Zh. Nevrol. Psikhiatrii, 1998, vol. 98, pp. 49–56.

    Google Scholar 

  11. Wonnapinij, P., Chinnery, P.F., and Samuels, D.C., Am. J. Hum. Genet., 2008, vol. 3, no. 5, pp. C. 582–93.

    Article  Google Scholar 

  12. Nikitina, L.P., Kuznetsova, N.S., Gomboeva, A.Ts., and Solov’eva, N.V., Zabaikal. Med. Vestnik, 2011, no. 2, pp. 184–184.

    Google Scholar 

  13. Egorova, L.A., Ezhov, M.V., Shiganova, G.M., and Postnov, A.Yu., Klinitsist, 2013, no. 2, pp. 6–6.

    Google Scholar 

  14. Prokhorova, N.S. and Demidenko, L.A., Tavrich. Med.-Biol. Vestnik, 2010, vol. 13, no. 4 (52), pp. 146–149.

    Google Scholar 

  15. Di Donato, S., J. Neurol., 2009, vol. 256, pp. 693–710.

    Article  PubMed  Google Scholar 

  16. Harbauer, A.B., Zahedi, R.P., Sickmann, A., Pfanner, N., and Meisinger, C., Cell Metab., 2014, vol. 4, pp. 357–372.

    Article  Google Scholar 

  17. Mazunin, I.O., Volod’ko, N.V., Starikovskaya, E.B., and Sukernik, R.I., Mol. Biol., 2010, vol. 44, no. 5, pp. 755–772.

    Article  CAS  Google Scholar 

  18. Patrushev, M.V., Kamenski, P.A., and Mazunin, I.O., Biochemistry (Moscow), 2014, vol. 79, no. 11, pp. 1151–1160.

    Article  CAS  Google Scholar 

  19. Doyle, S.R. and Chan, C.K., Human Gene Therapy, 2009, vol. 19, pp. 1348–1355.

    Google Scholar 

  20. Bacman, S.R., Williams, S.L., Pinto, M., Peralta, S., and Moraes, C.T., Nat. Med., 2013, vol. 19, no. 9, pp. 1111–1113.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Aanen, D.K., Spelbrink, J.N., and Beekman, M., Philos. Trans. R Soc. Lond. B Biol. Sci., 2014, vol. 369, no. 1646, p. 20130438.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Suzuki, T., Nagao, A., and Suzuki, T., Ann. Rev. Genet., 2011, vol. 45, pp. 299–329.

    Article  CAS  PubMed  Google Scholar 

  23. Yousif, L.F., Stewart, K.M., and Kelley, S.O., Chem. BioChem., 2009, vol. 10, pp. 1939–1950.

    CAS  Google Scholar 

  24. Tanaka, M., Borgeld, H.J., Zhang, J., Muramatsu, S., Gong, J.S., et al., J. Biomed. Sci., 2002, vol. 9, pp. 534–541.

    CAS  PubMed  Google Scholar 

  25. Bacman, S.R., Williams, S.L., Garcia, S., and Moraes, C.T., Gene Ther., 2010, vol. 17, pp. 713–720.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Isakova, E.P., Epova, E.Yu., Sekova, V.Yu., Trubnikova, E.V., Kudykina, Yu.K., Zyl’kova, M.V., Guseva, M.A., and Deryabina, Yu.I., Appl. Biochem. Microbiol., 2015, vol. 51, no. 3, pp. 336–341.

    Article  CAS  Google Scholar 

  27. Davidow, L.S., Apostolakos, D., O’Donnell, M.M., Proctor, A.R., Ogrydziak, D.M., Wing, R.A., Stasko, I., and DeZeeuw, J.R., Curr. Genet., 1985, vol. 10, pp. 39–48.

    Article  CAS  Google Scholar 

  28. Isakova, E.P., Deryabina, Yu.I., Sekova, V.Yu., Zyl’kova, M.V., Kudykina, Yu.K., and Teplova, V.V., Biol. Membr., 2015, vol. 32, nos. 5–6. DOI: 10.7868/S0233475515050059

    Google Scholar 

  29. Kovaleva, M.V., Sukhanova, E.I., Trendeleva, T.A., Zyl’kova, M.V., Ural’skaya, L.A., Popova, K.M., Saris, N.E., and Zvyagilskaya, R.A., J. Bioenerg. Biomembr., 2009, vol. 41, no. 3, pp. 239–249.

    Article  CAS  PubMed  Google Scholar 

  30. Smith, P., Krohn, R., Hermanson, G., Mallia, A., Gartner, F., Provenzano, M., et al., Anal. Biochem., 1985, vol. 150, pp. 76–85.

    Article  CAS  PubMed  Google Scholar 

  31. Laemmli, U.K., Nature, 1970, vol. 227, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

  32. Comte, C., Tonin, Y., Heckel-Mager, A.M., Boucheham, A., and Smirnov, A., Nucleic Acid Res., 2013, vol. 41, pp. 418–433.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Rodrigues-Posada, et al., Yeast, 2010, vol. 27, pp. 245–258.

  34. Gónzalez-Párraga, P., Alonso-Monge, R., Plá, J., and Argüelles, J.C., FEMS Yeast Res., 2010, no. 6, pp. 747–747.

    Article  Google Scholar 

  35. Briones-Martin-Del-Campo, M., Orta-Zavalza, E., Juarez-Cepeda, J., Gutierrez-Escobedo, G., Cañas-Villamar, I., Castaño, I., and De Las Peñas, A., Rev. Iberoam. Micol., 2014, vol. 31, no. 1, pp. 67–71.

    Article  PubMed  Google Scholar 

  36. Davidson, et al., Proc. Natl. Acad. Sci. USA, 1996, vol. 93, no. 10, pp. 5116–5121.

  37. Sekova, V.Yu., Gessler, N.N., Isakova, E.P., Antipov, A.N., Dergachev, D.I., Deryabina, Yu.I., and Trubnikova, E.V., Appl. Biochem. Microbiol., 2015, vol. 51, no. 6, pp. 649–654.

    Article  CAS  Google Scholar 

  38. Zvyagilskaya, R., Parchomenko, O., and Persson, B.L., IUBMB Life, 2000, no. 2, pp. 151–151.

    Article  Google Scholar 

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Fundamental Principles of Biotechnology, Federal Research Center, Russian Academy of Sciences, Moscow, 119071, Russia

    E. P. Isakova & Y. I. Deryabina

  2. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow, 142782, Russia

    O. A. Leonovich, M. V. Zylkova & Iu. K. Biriukova

Authors
  1. E. P. Isakova
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  2. Y. I. Deryabina
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  3. O. A. Leonovich
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  4. M. V. Zylkova
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  5. Iu. K. Biriukova
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Correspondence to E. P. Isakova.

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Original Russian Text © E.P. Isakova, Y.I. Deryabina, O.A. Leonovich, M.V. Zylkova, Iu.K. Biriukova, 2016, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2016, Vol. 52, No. 2, pp. 174–183.

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Isakova, E.P., Deryabina, Y.I., Leonovich, O.A. et al. Study of the Accumulation of Rec A from Bacillus subtilis in the Mitochondria of a Recombinant Strain of the Yeast Yarovia lipolytica . Appl Biochem Microbiol 52, 153–161 (2016). https://doi.org/10.1134/S0003683816020071

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  • DOI: https://doi.org/10.1134/S0003683816020071

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