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Isolation and characterization of Anthraquinone from Streptomyces sp. ERINLG-26 with anticancer activity Against adenocarcinoma cell line COLO320

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Abstract

Anticancer activity of 9,10-anthraquinone isolated from soil-derived (Doddabetta forest, Nilgiris, Tamil Nadu, India) filamentous bacterium Streptomyces sp. isolate ERINLG-26 was tested to explore the molecular mechanisms of action. The culture liquid ethyl acetate extract showed high cytotoxic activity against tested colorectal adenocarcinoma cell line COLO320. The ethyl acetate extract was fractionated by column chromatography over silica gel and preparative HPLC. The isolated compound, 9,10-anthraquinone, showed prominent cytotoxic activity in vitro against colorectal adenocarcinoma cell line COLO320. It showed 79.7% cytotoxicity at concentration of compound 300 μg/mL with IC50 value of 75 μg/mL. Treatment of the COLO320 cancer cells with isolated 9,10-anthraquinone significantly reduced cell proliferation and increased formation of fragmented DNA and apoptotic bodies. The expression of p53 and caspase-3 was up-regulated by 9,10-anthraquinone in colorectal adenocarcinoma cell line COLO320.

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References

  1. Raja, A. and Prabakarana, P., Am. J. Drug. Discovery Develop., 2011, vol. 1, no. 2, pp. 75–84.

    Article  Google Scholar 

  2. Cragg, G.M. and Newman, D.J., J. Ethnopharmacol., 2005, vol. 100, nos. 1–2, pp. 72–79.

    Article  CAS  PubMed  Google Scholar 

  3. Wyllie, A.H., Kerr, J.F.R., and Currie, A.R., Int. Rev. Cytol., 1980, vol. 68, p. 251.

    Article  CAS  PubMed  Google Scholar 

  4. Lee, H.Z., Hsu, S.L., Liu, M.C., and Wu, Ch.., Eur. J. Pharmacol., 2001, vol. 431, no. 3, pp. 287–295.

    Article  CAS  PubMed  Google Scholar 

  5. El–Deiry, W.S., Semin. Cancer Biol., 1998, vol. 8, no. 2, pp. 345–357.

    Article  PubMed  Google Scholar 

  6. Weng, J.R., Tsai, Ch.., Kulp, S.K., and Chen, C.S., Cancer Lett., 2008, vol. 262, no. 2, pp. 153–163.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Liebermann, D.A., Hoffman, B., and Vesely, D., Cell Cycle, 2007, vol. 6, no. 2, pp. 166–170.

    Article  CAS  PubMed  Google Scholar 

  8. Thornberry, N.A. and Lazebnik, Y., Science, 1998, vol. 281, no. 5381, pp. 1312–1316.

  9. Shirling, J.L. and Gottlieb, D., Int. J. Syst. Bacteriol., 1966, vol. 16, no. 3, pp. 313–340.

    Article  Google Scholar 

  10. Balachandran, C., Duraipandiyan, V., Balakrishna, K., and Ignacimuthu, S., Bioresour. Technol., 2012, vol. 112, pp. 83–90.

    Article  CAS  PubMed  Google Scholar 

  11. Balachandran, C., Arun, Y., Duraipandiyan, V., Ignacimuthu, S., Balakrishna, K., and Al-Dhabi, N.A., Appl. Biochem. Biotechnol., 2014, vol. 172, pp. 3513–3528.

    Article  CAS  PubMed  Google Scholar 

  12. Balachandran, C., Sangeetha, B., Duraipandiyan, V., Karunai Raj, M., Ignacimuthu, S., Al-Dhabi, N.A., et al., Chem. Biol. Interact., 2014, vol. 224, pp. 24–35.

    Article  CAS  PubMed  Google Scholar 

  13. Tamura, K., Dudley, J., Nei, M., and Kumar, S., Mol. Biol. Evol., 2007, vol. 24, no. 8, pp.1596–1599.

    Article  CAS  PubMed  Google Scholar 

  14. Balachandran, C., Duraipandiyan, V., Balakrishna, K., Sundaram, L.R, Vijayakumar, A., Ignacimuthu, S., and Al-Dhabi, N.A., Environ. Chem. Lett., 2013, vol. 11, no. 3, pp.303–308.

    Article  CAS  Google Scholar 

  15. Chomczynski, P. and Sacchi, N., Anal. Biochem., 1987, vol. 162, no. 1, pp.156–159.

    Article  CAS  PubMed  Google Scholar 

  16. Zhu, J., He, F., Hu, S., and Yu, J., Trends. Genet., 2008, vol. 24, no. 10, pp. 481–484.

    Article  CAS  PubMed  Google Scholar 

  17. Narayana, K.J.P., Kumar, K.G., and Vijayalakshmi, M., Ind. J. Microbiol., 2008, vol. 48, no. 3, pp. 331–336.

    Article  CAS  Google Scholar 

  18. Lee, H.Z., British J. Pharmacol., 2001, vol. 134, no. 5, pp. 1093–1103.

    Article  CAS  Google Scholar 

  19. Fisher, G.R., Brown, J.R., and Patterson, L.H., Free Rad. Res. Comm., 1990, vol. 11, pp. 117–125.

    Article  CAS  Google Scholar 

  20. Barasch, D., Zipori, O., Ringel, I., Ginsburg, I., Sam uni, A., and Katzhendler, J., Eur. J. Med. Chem., 1999, vol. 34, nos. 7–8, pp. 597–615.

    CAS  PubMed  Google Scholar 

  21. Mueller, S.O. and Stopper, H., Biochim. Biophys. Acta, 1999, vol. 1428, nos. 2–3, pp. 406–414.

    Article  CAS  PubMed  Google Scholar 

  22. Brown, J.M. and Wouters, B.G., Cancer. Res., 1999, vol. 59, no. 7, pp. 1391–1399.

    CAS  PubMed  Google Scholar 

  23. Nicholson, D.W., Cell Death Differ., 1999, vol. 6, no. 11, pp. 1028–1042.

    Article  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Division of Microbiology and Cancer Biology, Entomology Research Institute, Loyola College, Chennai, 600034, India

    C. Balachandran, V. Duraipandiyan, K. Balakrishna & S. Ignacimuthu

  2. Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia

    V. Duraipandiyan & N. A. Al-Dhabi

  3. Division of Bioinformatics, Entomology Research Institute, Loyola College, Nungambakkam, Chennai, Tamil Nadu, 600034, India

    A. Stalin

  4. Biozone Research Technologies Pvt. Ltd., Teynampet, Chennai, 600018, India

    F. Tilton

Authors
  1. C. Balachandran
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  2. V. Duraipandiyan
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  3. N. A. Al-Dhabi
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  4. A. Stalin
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  5. K. Balakrishna
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  6. S. Ignacimuthu
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  7. F. Tilton
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Correspondence to V. Duraipandiyan.

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Balachandran, C., Duraipandiyan, V., Al-Dhabi, N.A. et al. Isolation and characterization of Anthraquinone from Streptomyces sp. ERINLG-26 with anticancer activity Against adenocarcinoma cell line COLO320. Appl Biochem Microbiol 51, 522–529 (2015). https://doi.org/10.1134/S0003683815050038

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  • DOI: https://doi.org/10.1134/S0003683815050038

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