Abstract
Genes encoding truncated versions of bovine and chicken gamma interferon genes lacking predicted protease cleavage sites at the C-terminus were constructed and expressed in the yeast Pichia pastoris. The recombinant proteins possessed increased stability in comparison with the corresponding wild-type gamma interferons while retaining biological activity. The recombinant strains provide a useful tool for the purification of bovine and chicken gamma interferons for their use in veterinary applications.
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Original Russian Text © M.A. Tsygankov, A.E. Zobnina, M.V. Padkina, 2014, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 4, pp. 429–436.
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Tsygankov, M.A., Zobnina, A.E. & Padkina, M.V. Synthesis of recombinant gamma interferons resistant to proteolysis in the yeast Pichia pastoris . Appl Biochem Microbiol 50, 387–393 (2014). https://doi.org/10.1134/S0003683814040127
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DOI: https://doi.org/10.1134/S0003683814040127