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Purification, characterisation and coal depolymerisation activity of lignin peroxidase from Lenzitus betulina MTCC-1183

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Abstract

Lignin peroxidase from the culture filtrate of Lenzitus betulina MTCC-1183 has been purified to homogeneity using concentration by ultrafiltration and anion exchange chromatography on DEAE cellulose. The molecular weight of the purified lignin peroxidase using SDS-PAGE analysis was 43 kDa. Specific activity of the enzyme was 29.58 IU/mg. The K m values for veratryl alcohol and H2O2 for the purified enzyme were 54 and 81 μM, respectively. The k cat value of the purified enzyme was 2.3 s−1 using 3,4-dimethoxybenzyl alcohol as the substrate. The optimal conditions for the lignin peroxidase assay were detected at pH 2.4 and 22°C. Thermal stability of the purified enzyme has also been studied and its activation energy for deactivation was 287 kJ/mol. The purified lignin peroxidase depolymerised humic acid in presence of H2O2. Depolymerisation of coal by the L. betulina MTCC-1183 has been demonstrated using humic acid as a model of coal.

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References

  1. Edwards, S.L., Raag, R., Wariishi, H., Gold, M.H., and Poulos T.L., Proc. Natl. Acad. Sci. USA, 1993, vol. 90, no. 2, pp. 750–754.

    Article  PubMed  CAS  Google Scholar 

  2. Harley, B.S., Brodo, P.M.A., and Senior, P.J., in Proceedings of Royal Society Discussion Meeting on Utilisation of Lignocellulosic Wastes, Cambridge: Cambridge Univ. Press, 1988.

    Google Scholar 

  3. Catcheside, D.E.A. and Ralph, J.P., Appl. Microbiol. Biotechnol., 1999, vol. 52, no. 1, pp. 16–24.

    Article  CAS  Google Scholar 

  4. Eriksson, K.E. and Kirk, T.K., FEMS Microbiol. Rev., 1994, vol. 13, no. 2–3, pp. 351–364.

    Google Scholar 

  5. Bumpus, J.A., Tien, M., Wright, D., and Aust, S.D., Science, 1985, vol. 228, no. 4706, pp. 1434–1436.

    Article  PubMed  CAS  Google Scholar 

  6. Kwant, S.S. and Chang, J. K., Biotechnol. Lett., 1998, vol. 20, no. 6, pp. 569–572.

    Article  Google Scholar 

  7. Satwinder, S.M., Rajesh, G., Chand, P., and Kennedy, J.F., J. Chem. Technol. Biotechnol., 1998, vol. 73, no. 3, pp. 292–296.

    Article  Google Scholar 

  8. Levin, L., Papinutti, L., and Forchiassin, F., Biores. Technol., 2004, vol. 94, no. 2, pp. 169–176.

    Article  CAS  Google Scholar 

  9. Novotny, C., Svobodova, K., Erbanova, P., Cajthaml, T., Kasinath, A., Lang, E., and Sasek, V., Soil Biol. Biochem., 2004, vol. 36, no. 10, pp. 1545–1551.

    Article  CAS  Google Scholar 

  10. Uyama, H. and Kobayashi, S., Chemtech., 1999, vol. 29, no. 10, pp. 22–28.

    CAS  Google Scholar 

  11. Kumari, M., Yadav, R.S.S., and Yadav, K.D.S., Indian. J. Expt. Biol., 2002, vol. 40, no. 7, pp. 802–806.

    CAS  Google Scholar 

  12. Shanmugan, V. and Yadav, K.D.S., Indian. J. Microbiol., 1997, vol. 37, no. 15, pp. 105–106.

    Google Scholar 

  13. Shanmugan, V. and Yadav, K.D.S., Indian. J. Expt. Biol., 1996, vol. 34, pp. 1164–1165.

    Google Scholar 

  14. Patel, V.K., Yadav, R.S.S., and Yadav, K.D.S., Indian. J. Biotechnol., 2007, vol. 6, no. 4, pp. 553–556.

    CAS  Google Scholar 

  15. Tien, M. and Kirk, T.K., Methods in Enzymology, 1988, vol. 161, pp. 238–249.

    Article  CAS  Google Scholar 

  16. Gold, M.H. and Glenn, J.K., Methods in Enzymology, 1988, vol. 161, pp. 258–264.

    Article  CAS  Google Scholar 

  17. Coll, M.P., Fernandez-Abalos, J.M., Villomueva, J.R., Somtamaria, R., and Perez, P., Appl. Environ. Microbiol., 1993, vol. 59, no. 8, pp. 2607–2613.

    PubMed  CAS  Google Scholar 

  18. Werber, K. and Osborn, M., J. Biol. Chem., 1969, vol. 244, no. 16, pp. 4406–4412.

    Google Scholar 

  19. Morrissey, J.H., Anal. Biochem., 1981, vol. 117, no. 2, pp 307–310.

    Article  PubMed  CAS  Google Scholar 

  20. Fersht, A., Structure and Mechanism in Protein Science, New York: Freeman, 1998.

    Google Scholar 

  21. Hafrichter, M. and Fritsche, W., Appl. Microbiol. Biotechnol., 1996, vol. 46, no. 3, pp. 220–225.

    Article  Google Scholar 

  22. Wang, P., Hu, X., Cook, S., Begonia, M., Lee, S.K., and Hwang, H., World J. Microbiol. Biotechnol., 2008, vol. 24, no. 10, pp. 2205–2212.

    Article  Google Scholar 

  23. Wondrack, L., Scanto, M., and Wood, W.A., Appl. Biochem. Biotechnol., 1989, vol. 20–21, no. 1, pp. 765–780.

    Article  Google Scholar 

  24. Yadav, M., Singh, S.K., and Yadav, K.D.S., J. Wood Chem. Technol., 2009, vol. 29, no. 1, pp. 59–73.

    Article  CAS  Google Scholar 

  25. Nozomi, K., Yohru, S., and Kehchi, K., J. Biosci. Bioeng., 2002, vol. 93, no. 4, pp. 405–410.

    Google Scholar 

  26. Yadav, M., Yadav, P., and Yadav, K.D.S., Eng. Life Sci., 2009, vol. 9, no. 2, pp. 1–6.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. D.D.U. Gorakhpur University, Gorakhpur, 273 009, India

    M. Yadav, S. K. Singh & S. Yadava

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  1. M. Yadav
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  2. S. K. Singh
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  3. S. Yadava
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Correspondence to M. Yadav.

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Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2012, Vol. 48, No. 6, pp. 646–652.

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Yadav, M., Singh, S.K. & Yadava, S. Purification, characterisation and coal depolymerisation activity of lignin peroxidase from Lenzitus betulina MTCC-1183. Appl Biochem Microbiol 48, 583–589 (2012). https://doi.org/10.1134/S0003683812050146

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  • DOI: https://doi.org/10.1134/S0003683812050146

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