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Spectrophometric analysis of volatile compounds in microorganisms

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Abstract

A simple modification of a spectrophometric method was proposed for the rapid detection of microorganisms based on their ability either to excrete or to absorb volatile compounds. The method provides the possibility of contactless control for bacterial growth at a concentration above 107 cells/ml. In addition, the method allows discriminating mutants of the fungus Neurospora crassa defective in the nitrogen metabolism from the wild type strains. It is likely that nitrite reductase and nitrate reductase enzymes regulated by the nit-2 and nit-6 genes are involved in formation of the water soluble volatile compounds of this organism.

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Authors and Affiliations

  1. State Scientific Center of Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russia

    A. G. Voloshin, S. G. Besaeva & S. G. Ignatov

  2. Bach Institute of Biochemistry, Russian Academy of Science, Moscow, 119071, Russia

    S. Yu. Filippovich & G. P. Bachurina

Authors
  1. A. G. Voloshin
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  2. S. Yu. Filippovich
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  3. G. P. Bachurina
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  4. S. G. Besaeva
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  5. S. G. Ignatov
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Corresponding author

Correspondence to S. G. Ignatov.

Additional information

Original Russian Text © A.G. Voloshin, S.Yu. Filippovich, G.P. Bachurina, S.G. Besaeva, S.G. Ignatov, 2010, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2010, Vol. 46, No. 3, pp. 331–335.

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Voloshin, A.G., Filippovich, S.Y., Bachurina, G.P. et al. Spectrophometric analysis of volatile compounds in microorganisms. Appl Biochem Microbiol 46, 303–306 (2010). https://doi.org/10.1134/S0003683810030099

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  • DOI: https://doi.org/10.1134/S0003683810030099

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