Abstract
This study evaluates dynamic changes in levels of Hsp70s and products of lipid peroxidation due to hyperthermia in the laboratory-raised freshwater pulmonate mollusk Stagnicola corvus (Gmelin, 1791). It is concluded that acute hyperthermia in S. corvus leads to the activation of nonspecific mechanisms of stress adaptation, which is manifested in an elevated Hsp70s level in the beginning of exposure, followed by a reduction in protein concentration, and the further preservation of the Hsp70 at a level exceeding the constitutive forms. At the same time, the level of LPO products tends to decrease, which indicates strengthening of the antioxidant defense processes in response to stress.
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Aksenov-Gribanov, D.V., Shakhtanova, N.S., Vereshchagina, K.P., Shchapova, E.P., and Lubyaga, Yu.A., The effect of temperature increase on the activity of thermoresistance mechanisms and the energy metabolism in Baikal and Palaearctic gastropods, Materialy VI Vserossiiskoi nauchnogo kongressa molodokh uchenykh s mezhdunarodnym uchastiem, Irkutsk, 19–28 avgusta 2013 g., Tezisy dokladov (Proc. VI All-Russ. Sci. Congr. of Young Scientists with International Participation, Irkutsk, August 19–28, 2013, Abstracts of Papers), Irkutsk, 2013, pp. 129–131.
Axenov-Gribanov, D.V., Bedulina, D.S., Shatilina, Z.M., Lubyaga, Y.A., Vereshchagina, K.P., and Timofeyev, M.A., A cellular and metabolic assessment of the thermal stress responses in the endemic gastropod Benedictia limnaeoides ongurensis from Lake Baikal, Comp. Biochem. Physiol., Part B: Biochem. Mol. Biol., 2014, vol. 167, pp. 16–22.
Axenov-Gribanov, D., Vereshchagina, K., Lubyaga, Y., Gurkov, A., Bedulina, D., Shatilina, Z., Homich, A., Golubev, A., and Timofeyev, M., Stress response at the cellular and biochemical levels indicates the limitation of the environmental temperature range for Eastern Siberia populations of the common gastropod Limnaea stagnalis, Malacologia, 2015, vol. 59, no. 1, pp. 33–44.
Baby, J., Jency, G., and Jeevitha, M.V., Impact of heavy metals and Hsp response, Int. J. Biosci., 2012, vol. 2, no. 9, pp. 51–64.
Bedulina, D.S., Evgen’ev, M.B., Timofeyev, M.A., Protopopova, M.V., Garbuz, D.G., Pavlichenko, V.V., Luckenbach, T., Shatilina, Z.M., Axenov-Gribanov, D.V., Gurkov, A.N., Sokolova, I.M., and Zatsepina, O.G., Expression patterns and organization of the hsp70 genes correlate with thermotolerance in two congener endemic amphipod species (Eulimnogammarus cyaneus and E. verrucosus) from Lake Baikal, Mol. Ecol., 2013, vol. 22, pp. 1416–1430.
Bers, G. and Garfin, D., Protein and nucleic acid blotting and immunobiochemical detection, BioTechniques, 1985, vol. 3, pp. 276–288.
Bolotov, I.N., Besplaya, Yu.V., and Usacheva, O.V., Ecology and evolution of hydrobionts in hot springs of the subarctic and arctic: formation of similar assemblages, adaptation of species, and microevolutionary processes, Biol. Bull. Rev., 2012, vol. 2, no. 4, pp. 340–348.
Borovikov, V.P., Isskustvo analiza dannykh na komp’yutere (The Art of Computer Data Analysis), St. Petersburg: Statistica, 2003.
Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, vol. 72, no. 1, pp. 248–254.
Cantinha, R., Borrely, S., Oguiura, N., Polpo, A., Pereira, C.A.B., and Nakano, E., Thermotolerance and induction of HSP70 in Biomphalaria glabrata, Greener J. Cell Anim. Biol., 2013, vol. 2, no. 3, pp. 1–7.
Clark, M.S., Geissler, P., Waller, C., Fraser, K.P., Barnes, D.K., and Peck, L.S., Low heat-shock thresholds in wild Antarctic inter-tidal limpets (Nacella concinna), Cell Stress Chaperones, 2008, vol. 13, no. 1, pp. 51–58.
Di Lorenzo, T., Cannicci, S., Spigoli, D., Cifoni, M., Baratti, M., and Galassi, D.M., Bioenergetic cost of living in polluted freshwater bodies: respiration rates of the cyclopoid Eucyclops serrulatus under ammonia-N exposures, Fundam. Appl. Limnol., 2016, vol. 188, no. 2, pp. 147–156.
Dong, Y., Miller, L.P., Sanders, J.G., and Somero, G.N., Heat-shock protein 70 (Hsp70) expression in four limpets of the genus Lottia: interspecific variation in constitutive and inducible synthesis correlates with in situ exposure to heat stress, Biol. Bull., 2008, vol. 215, no. 2, pp. 173–181.
Dongwu, L. and Zhiwei, C., The expression and induction of heat shock proteins in mollusks, Protein Peptide Lett., 2013, vol. 20, no. 5, pp. 602–606.
Gnatishina, L.L., Fal’fushinskaya, G.I., Golubev, A.P., Dallinger, R., and Stoliar, O.B., Role of metallothioneins in adaptation of Lymnaea stagnalis (Mollusca: Pulmonata) to environment pollution, Hydrobiol. J., 2011, vol. 47, no. 5, pp. 56–66.
Golubev, A.P., Bodilovskaya, O.A., Khomich, A.S., Korotchikova, N.V., Vereshchagina, K.P., Lubyaga, Y.A., Shchapova, E.P., Shatilina, Z.M., and Axenov-Gribanov, D.V., The influence of trematode invasion on the thermoresistance of Lymnaea stagnalis (Gastropoda, Pulmonata) population from the floodplain reservoir of Angara River, J. Stress Physiol. Biochem., 2015, vol. 11, no. 2, pp. 28–39.
Gueraud, F., Atalay, M., Bresgen, N., Cipak, A., Eckl, P.M., Huc, L., Jouanin, I., Siems, W., and Uchida, K., Chemistry and biochemistry of lipid peroxidation products, Free Radical Res., 2010, vol. 44, no. 10, pp. 1098–1124.
Hochachka, P.W. and Somero, G.N., Biochemical Adaptation, Princeton: Princeton Univ. Press, 2014.
Il’ina, T.N., Ilyukha, V.A., Baishnikova, I.V., Belkin, V.V., Sergina, S.N., and Antonova, E.P., Comparative analysis of the antioxidant system of semi-aquatic and terrestrial mammals, Tr. Karel. Nauch. Tsentr, Ross. Akad. Nauk, 2016, no. 6, pp. 16–24s.
Jakubic, B., Koperski, P., and Lewandowski, K., Diversity of mollusks in lowland river-like system: lentic versus lotic patches, Pol. J. Ecol., 2014, vol. 62, pp. 335–348.
Kantor, Y.I., Schileyko, A.A., Vinarski, M.V., and Sysoev, A.V., Catalogue of the continental mollusks of Russia and adjacent territories, 2011. http://www.ruthenica.com/documents/Continental_Russian_molluscs_ver1-0.
Khyshiktuev, B.S., Khyshiktueva, N.A., and Ivanov, V.N., Determination of the products of lipid peroxidation in the condensate of exhaled air and their clinical significance, Klin. Lab. Diagn., 1996, no. 3, pp. 13–15.
Koshelev, A.V. and Ovsepyan, M.S., Survival and behavioral changes of Potamopyrgus antipodarum in the salinity gradient, Nauk. Zap. Ternopol. Nats. Pedagog. Univ., Ser.: Biol., 2012, no. 2 (51), pp. 148–15
Laemmli, U.K., Cleavage of structural proteins during the assemble of the head bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685.
Laenko, T.M., Fauna vodnykh mollyuskov Belarusi (Fauna of Aquatic Mollusks of Belarus), Minsk: Belarusskaya Navuka, 2012.
Men’shchikova, E.B., Lankin, V.Z., Zenkov, N.K., Bondar’, I.A., Krugovykh, N.F., and Trufakin, V.A., Okislitel’nyi stress. Prooksidanty i antioksidanty (Oxidative Stress. Prooxidants and Antioxidants), Moscow: Slovo, 2006.
Morimoto, R.I. and Nollen, E.A.A., The heat shock response and the stress of misfolded proteins, in Handbook of Cell Signaling, Amsterdam: Elsevier, 2009, pp. 267–274.
Nguyen, K.D.T., Morley, S.A., Lai, C.H., Clark, M.S., Tan, K.S., Bates, A.E., and Peck, L.S., Upper temperature limits of tropical marine ectotherms: global warming implications, PLoS One, 2011, vol. 6, no. 12, p. e29340.
Parris, K.M., Ecology of Urban Environments, Chichester: Wiley, 2016.
Panin, V.F., Zashchita biosfery ot energeticheskikh vodeistvii: konspekt lektsii (Biosphere Protection from Energetic Impact: Lectures), Tomsk: Tomsk. Pedagog. Univ., 2009.
Podlipaeva, Yu.I., Gudkov, A.V., and Berger, V.Ya., The content dynamics of 70 kDa stress protein during adaptation of mollusks Mytilus edulis L. to reduced salinity, Tsitologiya, 2016, vol. 58, no. 7, pp. 562–566.
Prie, V., von Proschwitz, T., and Seddon, M.B., Stagnicola corvus, The IUCN Red List of threatened species, version 2014.3, 2011. http://dx.doi.org/.doi10.2305/IUCN.UK.2011-2.RLTS.T155596A4805799.en
The HSP70 Molecular Chaperone Machines, Mayer, M.P. and Goloubinoff, P., Eds., Lausanne: Frontiers Media, 2017.
Wang, L., Yang, C., and Song, L., The molluscan HSP70s and their expression in hemocytes, Inf. Syst. J., 2013, vol. 10, pp. 77–83.
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Original Russian Text © A.S. Khomich, A.P. Golubev, D.V. Axenov-Gribanov, O.A. Bodilovskaya, Y.A. Shirokova, Y.V. Loshakova, Y.A. Lubyaga, Z.M. Shatilina, 2018, published in Sibirskii Ekologicheskii Zhurnal, 2018, No. 1, pp. 79–88.
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Khomich, A.S., Golubev, A.P., Axenov-Gribanov, D.V. et al. The Effect of Acute Hiperthermia on Levels of Hsp70 and Lipid Peroxidation in Laboratory-Cultured Pulmonate Mollusk Stagnicola corvus. Contemp. Probl. Ecol. 11, 64–71 (2018). https://doi.org/10.1134/S1995425518010067
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DOI: https://doi.org/10.1134/S1995425518010067