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Comparative Analysis of Physicochemical Characteristics of Fluorophore Groups of Lipofuscin Granules from Retinal Pigment Epithelium Cells of Human Cadaver Eyes in Normal and Pathology

  • CHEMICAL PHYSICS OF BIOLOGICAL PROCESSES
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An Erratum to this article was published on 01 June 2022

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Abstract

A comparative study of individual groups of bisretinoids and their oxidation and degradation products in chloroform extracts from cells of the retinal pigment epithelium (RPE) of human cadaver eyes without signs of pathology (norm) and in the case of visualized age-related macular degeneration is carried out. Fluorescence analysis and high-performance liquid chromatography-mass spectrometry show that with age-related macular degeneration, an increased content of oxidation and degradation products of bisretinoids are observed in comparison with the norm. The results obtained allow us to conclude that the listed methods can be used in the preclinical diagnosis of degenerative diseases of the retina and the RPE of the human eye.

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Funding

This study was financially supported by a grant from the Ministry of Science and Higher Education of the Russian Federation (agreement no. 075-15-2020-773). The equipment and resources of the Center for Collective Use of the Institute of Biochemical Physics, Russian Academy of Sciences “New Materials and Technologies” were used in this study.

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Authors and Affiliations

  1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia

    M. A. Yakovleva, A. Sh. Radchenko, A. A. Kostyukov, A. S. Kononikhin, V. A. Kuzmin, T. B. Feldman & M. A. Ostrovsky

  2. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    V. V. Chagovets

  3. Skolkovo Institute of Science and Technology, Moscow, Russia

    A. S. Kononikhin & E. N. Nikolaev

  4. Fyodorov Eye Microsurgery, Ministry of Health of the Russian Federation, Moscow, Russia

    M. Kh. Khubetsova & S. A. Borzenok

  5. Moscow State University, Moscow, Russia

    T. B. Feldman & M. A. Ostrovsky

Authors
  1. M. A. Yakovleva
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  2. A. Sh. Radchenko
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  3. A. A. Kostyukov
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  4. V. V. Chagovets
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  5. A. S. Kononikhin
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  6. M. Kh. Khubetsova
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  7. S. A. Borzenok
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  8. V. A. Kuzmin
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  9. E. N. Nikolaev
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  10. T. B. Feldman
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  11. M. A. Ostrovsky
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Corresponding author

Correspondence to M. A. Yakovleva.

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The original online version of this article was revised: correct article title.

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Yakovleva, M.A., Radchenko, A.S., Kostyukov, A.A. et al. Comparative Analysis of Physicochemical Characteristics of Fluorophore Groups of Lipofuscin Granules from Retinal Pigment Epithelium Cells of Human Cadaver Eyes in Normal and Pathology. Russ. J. Phys. Chem. B 16, 90–96 (2022). https://doi.org/10.1134/S199079312201033X

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  • DOI: https://doi.org/10.1134/S199079312201033X

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