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Highly active fractions of the medicinal leech recombinant destabilase-lysozyme

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Abstract

Fractionation of the highly purified but low active recombinant protein destabilase-lysozyme (Dest-Lys) by cation exchange chromatography on a TSK CM 3-SW chromatography the non-active fraction (IV) containing 90% of total protein has been separated. Fractions I, II, and III contained proteins with lysozyme and isopeptidase activities and their lysozyme activity correlated with the activity of native Dest-Lys. However, the ratio of lysozyme and isopeptidase activities differed in these fractions; maximal lysozyme activity was found in fraction III, while maximal isopeptidase activity was associated with fraction I. Possible regulation of different functions of Dest-Lys is discussed in the context of formation of its various complexes.

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References

  1. Baskova, I.P. and Nikonov, G.I., Biokhimiya, 1985, vol. 50, pp. 424–431.

    CAS  Google Scholar 

  2. Zavalova, L., Lukyanov, S., Baskova, I., Snezhkov, E., Akopov, S., Berezhnoy, S., Bogdanova, E., Barsova, E., and Sverdlov, E., Mol. Gen. Genet., 1996, vol. 253, pp. 20–25.

    Article  CAS  Google Scholar 

  3. Zavalova, L.L., Baskova, I.P., Lukyanov, A.V., Sass, A.V., Snezkov, E.V., Akopov, S.B., Artamonova, I.I., Archipova, V.S., Nesmeyanov, V.A., Kozlov, D.G., et al., Biochim. Biophys. Acta, 2000, vol. 1478, pp. 69–77.

    Article  CAS  Google Scholar 

  4. Zavalova, L.L., Yudina, T.G., Artamonova, I.I., and Baskova, I.P., Chemotherapy, 2006, vol. 52, pp. 158–160.

    Article  CAS  Google Scholar 

  5. Zavalova, L.L., Lazarev, V.N., Levitskii, S.A., Yudina, T.G., and Baskova, I.P., Biochemistry (Moscow), 2010, vol. 75, pp. 1314–1324.

    Article  Google Scholar 

  6. Baskova, I.P., Zavalova, L.L., Basanova, A.V., and Grigor’eva, O.V., Bioorgan. Khim., 1999, vol. 25, pp. 435–438.

    CAS  Google Scholar 

  7. Bradford, M.M., Anal. Biochem., 1976, vol. 72, pp. 248–254.

    Article  CAS  Google Scholar 

  8. Baskova, I.P. and Nikonov, G.I., Blood Coagulation and Fibrinolysis, 1991, vol. 2, pp. 167–172.

    Article  CAS  Google Scholar 

  9. Goto, T., Abe, Y., Kakuta, Y., Takeshita, K., Imoto, T., and Ueda, T., J. Biol. Chem., 2007, vol. 282, pp. 27459–27467.

    Article  CAS  Google Scholar 

  10. Zavalova, L.L., Antipova, N.V., Fadeeva, Yu.I., Pavlyukov, M.S., Pletneva, N.V., Pletnev, V.Z., and Baskova, I.P., Bioorgan. Khim., 2012, vol. 38, pp. 229–233.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    Yu. I. Fadeeva, N. V. Antipova & L. L. Zavalova

  2. Biological Faculty, Lomonosov Moscow State University, Moscow, 119991, Russia

    I. P. Baskova

Authors
  1. Yu. I. Fadeeva
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  2. N. V. Antipova
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  3. I. P. Baskova
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  4. L. L. Zavalova
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Corresponding author

Correspondence to I. P. Baskova.

Additional information

Original Russian Text © Yu.I. Fadeeva, N.V. Antipova, I.P. Baskova, L.L. Zavalova, 2014, published in Biomeditsinskaya Khimiya.

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Fadeeva, Y.I., Antipova, N.V., Baskova, I.P. et al. Highly active fractions of the medicinal leech recombinant destabilase-lysozyme. Biochem. Moscow Suppl. Ser. B 8, 69–72 (2014). https://doi.org/10.1134/S199075081401003X

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  • DOI: https://doi.org/10.1134/S199075081401003X

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