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The role of induction and inhibition of nitric oxide synthesis in regulation of blood neutrophils cell death during oxidative imbalance

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Abstract

Modeling of oxidative stress in vitro with 5 mM H2O2 has demonstrated a protective role of nitric oxide in realization of constitutional blood neutrophil cell death. The NO synthase inductor, L-arginine, and the inhibitor of nitric oxide synthesis, L-NAME, influenced the amount of annexin-positive cells, the content of Bax protein, reactive oxygen species, cyclic nucleotides, and calcium homeostasis in neutrophils under conditions realizing programmed death during oxidative stress in vitro and under acute inflammation. During oxidative stress L-arginine normalized an increased intracellular Ca2+ level and the cAMP/cGMP ratio by increasing the cGÌP level, stabilized metabolism and prolonged neutrophil lifetime. During acute inflammation NO induction was insufficient for limitation of Ca2+ release into cytosol and for onset of the apoptotic effect; blockade of NO synthesis deteriorated this situation by activating neutrophil apoptosis due to the sharp increase in the Ca2+ content and reduction of cytosolic cyclic nucleotides. The protective effect of NO on neutrophil cell death during oxidative imbalance was not associated with regulation of the proapoptotic protein Bax.

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Authors and Affiliations

  1. Department of Fundamental Basics of Clinical Medicine, Siberian State Medical University, Moskovky tract 2, Tomsk, 634050, Russia

    N. V. Ryazantseva, Yu. V. Starikov & V. A. Bychkov

  2. Department of Biochemistry and Molecular Biology, Siberian State Medical University, Moskovky tract 2, Tomsk, 634050, Russia

    T. V. Zhavoronok & E. A. Stepovaya

Authors
  1. N. V. Ryazantseva
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  2. T. V. Zhavoronok
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  3. E. A. Stepovaya
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  4. Yu. V. Starikov
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  5. V. A. Bychkov
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Correspondence to T. V. Zhavoronok.

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Original Russian Text © N.V. Ryazantseva, T.V. Zhavoronok, E.A. Stepovaya, Yu.V. Starikov, V.A. Bychkov, 2011, published in Biomeditsinskaya Khimiya.

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Ryazantseva, N.V., Zhavoronok, T.V., Stepovaya, E.A. et al. The role of induction and inhibition of nitric oxide synthesis in regulation of blood neutrophils cell death during oxidative imbalance. Biochem. Moscow Suppl. Ser. B 5, 197–202 (2011). https://doi.org/10.1134/S1990750811020156

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  • DOI: https://doi.org/10.1134/S1990750811020156

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