Abstract
New recombinant carriers—modular nanotransporters (MNTs)—with N-terminal ligand module to the epidermal growth factor receptor (EGFR) were developed and characterized. Human epidermal growth factor (hEGF) and antibody-like protein Z1907 were used as a ligand module. We demonstrated that MNTs are able to internalize in a receptor-specific manner into the target cancer cells and to accumulate in the target cell nuclei. Conjugation of MNTs with the Auger electron emitter 111In significantly enhanced the cytotoxic effect of 111In on the target cells. It was found that the transfer of EGF from the C-terminus to the N-terminus of the MNT enhanced the proliferation of target cells, whereas the use of Z1907 did not have a similar effect.
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ACKNOWLEDGMENTS
This study was performed using the equipment of the Core Facility of the Institute of Gene Biology, Russian Academy of Sciences and Moscow State University Development Program.
Funding
The study was supported by the Russian Science Foundation (project no. 17-14-01304).
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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
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Translated by M. Batrukova
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Karyagina, T.S., Ulasov, A.V., Rosenkranz, A.A. et al. New Recombinant Carriers Binding Specifically to the Epidermal Growth Factor Receptor. Dokl Biochem Biophys 490, 22–24 (2020). https://doi.org/10.1134/S1607672920010081
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DOI: https://doi.org/10.1134/S1607672920010081