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Equipment for investigations of biological nanostructures by diffraction methods using synchrotron radiation

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Abstract

This paper reports on the experimental and methodical developments intended for the small-angle time-resolved station DICSI (“diffraction movie“) at the K1.3a beamline of the Siberia-2 storage ring at the Russian Research Centre “Kurchatov Institute.“ The principles of operation of the optical geometric and detecting systems, as well as the control and recording system, as applied to the investigation of the dynamics of biological nanostructures, have been considered in detail. A new version of the two-coordinate detecting system based on the designed highly effective fluorescent screen (Gd2O2S: Tb) and a CSDU-429 digital camera has been developed. The advantage of this recording system over the Image Plate detecting system in phenomenological investigations is that the optical images obtained on X-ray fluorescent screens with the use of cooled CCD/CMOS arrays can be directly entered into a computer. The detecting system has been tested on different samples by small- and wide-angle X-ray diffraction methods with the use of synchrotron radiation of the VEPP-3 storage ring at the Siberian Synchrotron Center (Novosibirsk, Russia) and the Siberia-2 storage ring at the Kurchatov Center for Synchrotron Radiation and Nanotechnology (Moscow, Russia). Different modifications of original two-coordinate recording systems have been used in systematic studies of the structural dynamics of biological tissues.

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References

  1. Vazina, A.A., Gerasimov, V.S., Zheleznaya, L.A., Savelyev, V.B., Srebnitskaya, L.K., Frank, G.M., Kulipanov, G.N., Skrinskii, A.N., Khlestov V.B., and Sheromov, M.A., Application of Synchrotron Radiation for Investigating Large Periods of the Structure of Biopolymers, Appar. Metody Rentgenovskogo Anal., 1975, no. 19, pp. 73–81.

  2. Korneev, V.N., Sergienko, P.M., Matyushin, A.M., Shlektarev, V.A., Ariskin, N.I., Shishkov, V.I., Gorin, V.P., Sheromov, M.A., Aul’chenko, V.M., Zabelin, A.V., Stankevich, V.G., Yudin, L.I., and Vazina, A.A., Current Status of the Small-Angle Station at Kurchatov Center of Synchrotron Radiation, Nucl. Instrum. Methods Phys. Res., Sect. A, 2005, vol. 543, pp. 368–374.

    Article  CAS  ADS  Google Scholar 

  3. Vazina, A.A., Bras, W., Dolbnya, I.P., Korneev, V.N., Lanina, N.F., Matyushin, A.M., Sergienko, P.M., and Zabelin, A.V., Peculiarities of Human Hair Structural Dynamics, Nucl. Instrum. Methods Phys. Res., Sect. A, 2005, vol. 543, pp. 153–157.

    Article  CAS  ADS  Google Scholar 

  4. Aul’chenko, V.M., Vazina, A.A., Galimov, P.V., Korneev, V.N., Maevskii, E.I., Matyushin, A.M., Tuzikov, F.V., and Tuzikova, N.A., Determination of Some Structural and Dispersion Characteristics of Perftoran Emulsions by the Small-Angle X-Ray Scattering, Nucl. Instrum. Methods Phys. Res., Sect. A, 2005, vol. 543, pp. 158–160.

    Article  ADS  Google Scholar 

  5. Vazina, A.A., Budantsev, A.Yu., Bras, W., Deshcherevskaya, N.P., Dolbnya, I.P., Gadzhiev, A.M., Korneev, V.N., Lanina, N.F., Letyagin, V.P., Maevsky, E.I., Matyushin, A.M., Podolsky, I.Ya., Samsonova, M.V., Sergienko, P.M., Simonova, N.B., Stankevich, V.G., Trunova, V.A., Vavilov, V.M., Chernyaev, A.L., Sharafutdinov, M.R., and Sheromov, M.A., X-Ray Diffraction and Spectral Studies of Biological Native and Modified Tissues, Nucl. Instrum. Methods Phys. Res., Sect. A, 2005, vol. 543, pp. 297–301.

    Article  CAS  ADS  Google Scholar 

  6. Vazina, A.A., Sergienko, P.M., Korneev, V.N., et al., Structural Nanoinvariant of Proteoglycan of Mucus and Extracellular Matrix of Biological Tissues, in Proceedings of the Alpbach Workshop “Coiled-Coils, Collagen, and Co-Proteins: IV,” Alpbach, Austria, Alpbach, 2005, p. 36.

  7. Vazina, A., Bras, W., Dolbnya, I., et al., New Insight into the High Elasticity of the Multidomain Giant Protein Titin within the Framework of the Physics of Structural Transitions in “Crystalline Polymers” during Stretching, in Abstracts of Papers of the XIV London Muscle Conference, London, United Kingdom, London, 2006, p. 11.

  8. Vazina, A.A., Lanina, N.F., Korneev, V.N., Matyushin, A.M., Zabelin, A.V., Nayda, O.V., Maevsky, E.I., and Polyakova, E.P., Nanostructure Ordering of Proteoglycans of Some Biological Objects, in Proceedings of the Topical Meeting of the European Ceramic Society “Structural Chemistry of Partially Ordered Systems, Nanoparticles, and Nanocomposites,” St. Petersburg, Russia, 2006, St. Petersburg, 2006 (Glass Phys. Chem., 2007, vol. 33, no. 3, pp. 294–301).

  9. Korneev, V.N., Zabelin, A.V., Shlektarev, V.A., Matyushin, A.M., Vazina, A.A., Nayda, O.V., Aul’chenko, V.M., Tolochko, B.L., and Sharafutdinov, M.R., New Time-Resolved Small-Angle Station with the Use of Synchrotron Radiation of the Siberia-2 Storage Ring for Investigation of Biological Structures, in Proceedings of the Topical Meeting of the European Ceramic Society “Structural Chemistry of Partially Ordered Systems, Nanoparticles, and Nanocomposites,” St. Petersburg, Russia, 2006, St. Petersburg, 2006 (Glass Phys. Chem., 2007, vol. 33, no. 3, pp. 266–270).

  10. Vazina, A.A., Lanina, N.F., Vasilieva, A.A., et al., The Principles of Nanostructural Organization of Rod-Like Biological Structures Such as the Multidomain Muscle Protein Titin, Thesis of the Workshop on Molecular Motors, Alpbach, Austria, 2007, Alpbach, 2007, p. 11.

  11. Sergienko, P.M., Gerasimov, V.S., Vazina, A.A., Korneev, V.N., Kulipanov, G.N., Aulchenko, V.M., and Stankevich, V.G., Status of Time-Resolved Small-Angle X-Ray Diffraction Technique at Siberian and Moscow SR Centres, in Abstracts of Papers of the XVIII International Union of Crystallography (IUCr) Congress and General Assembly, Glasgow, Scotland, 1999, Glasgow, 1999, p. 225.

  12. Vazina, A.A., Biological Application of Synchrotron Radiation: From Artem Alikhanyan to Nowadays, in Brilliant Light in Life and Material Sciences, Tsakanov, V. and Wiedemann, H., Eds., Dordrecht, The Netherlands: Springer, 2007, pp. 121–131.

    Chapter  Google Scholar 

  13. Vazina, A.A., Lanina, N.F., Korneev, V.N., et al., X-Ray Fluorescence and Small-Angle Diffraction Study of Proteoglycan Structures of Mucins and Extracellurar Matrix of Biological Tissues, in Abstracts of the Conference “Fibrous Proteins: Transforming Structural Knowledge into New Materials,” Melbourne, Australia, 2008, Melbourne, 2008, p. 31.

  14. Aulchenko, V.M., Bukin, M.A., Velikzhanin, Yu.S., Gaponenko, Ya.V., Dubrovin, M.S., Titov, V.M., Ancharov, A.I., Gaponov, Yu.A., Evdokov, O.V., Tolochko, B.P., and Sharafutdinov, M.R., Fast, Parallax-Free, One-Coordinate X-Ray Detector OD3, Nucl. Instrum. Methods Phys. Res., Sect. A, 1998, vol. 405, pp. 269–273.

    Article  CAS  ADS  Google Scholar 

  15. Korneev, V.N., Ariskin, N.I., Aulchenko, V.M., Bukin, M.A., Vazina, A.A., Gadzhiev, A.M., Gerasimov, V.S., Matyushin, A.M., Sergienko, P.M., Stankevich, V.G., Titov, V.V., Sheromov, M.A., Shishkov, V.I., and Yudin, L.I., The Station DICSI at KCSR “Siberia-2” for Time-Resolved Investigation of Dynamic Structure, Poverkhnost’, 2004, no. 10, pp. 24–27.

  16. Korneev, V.N., Shlektarev, V.A., Zabelin, A.V., Medvedev, B.I., Sharafutdinov, M.R., Tolochko, V.P., Sheromov, M.A., Kondratyev, V.I., Umnov, A.I., and Vazina, A.A., Elaboration of Station DICSI at KCSR and NT for Studies on Structural Dynamics of Biologic Objects, Nucl. Instrum. Methods Phys. Res., Sect. A, 2007, vol. 575, pp. 134–139.

    Article  CAS  ADS  Google Scholar 

  17. Korneev, V.N., Sergienko, P.M., Shlektarev, V.A., Aul’chenko, V.M., Bukin, M.A., Titov, V.M., Tolochko, B.P., Sharafutdinov, M.R., Zabelin, A.V., Litvinov, E.I., Matyushin, A.M., Stankevich, V.G., Sheromov, M.A., Naida, O.V., and Vazina, A.A., DICSI Station at KCSR and NT: Determination of Optimal Requirements to the Formation of an SR Beam Using Cylindrical X-Ray Optical Zoom Lenses, Poverkhnost’, 2007, no. 2, pp. 57–63 [J. Surf. Invest. (Engl. transl.), 2007, vol. 1, no. 1, pp. 99–104].

  18. Korneev, V.N., Shlektarev, V.A., Aul’chenko, V.M., Sharafutdinov, M.R., Tolochko, V.P., Zabelin, A.V., Naida, O.V., and Vazina, A.A., High-Time Resolution Radiographic Technique for Studying Nanostructures with the Use of Synchrotron Radiation, Izv. Akad. Nauk, Ser. Fiz., 2008, vol. 72, no. 2, pp. 214–217 [Bull. Russ. Acad. Sci.: Phys. (Engl. transl.), 2008, vol. 72, no. 2, pp. 197–200].

    CAS  Google Scholar 

  19. Korneev, V.N. and Gerasimov, V.S., Technique for Adjusting a Small-Angle X-Ray Chamber in Synchrotron Radiation, USSR Inventor’s Certificate no. 1245969, Byull. Izobret., 1986, no. 27, p. 5.

  20. Korneev, V.N., Shamarov, A.M., Gerasimov, V.S., et al., A Device for Adjusting the Diaphragm for Synchrotron X-Ray Radiation, USSR Inventor’s Certificate no. 1 521 134, Byull. Izobret., 1989, no. 41, p. 7.

  21. Savel’ev, V.B., Vazina, A.A., Sergienko, P.M., Fel’dman, I.G., and Khlestov, V.B., Small-Angle X-Ray Chamber for Investigations with Synchrotron Radiation, Prib. Tekh. Eksp., 1981, no. 4, pp. 213–216.

  22. Vazina, A.A., Gadzhiev, A.M., Sergienko, P.M., Gerasimov, V.S., and Korneev, V.N., Time-Resolved Small-Angle X-Ray Diffraction from Contracting Muscle, Rev. Sci. Instrum., 1989, vol. 60, no. 7, pp 2350–2353.

    Article  ADS  Google Scholar 

  23. Aul’chenko, V.M., Bukin, M.A., Vazina, A.A., Gadzhiev, A.M., Korneev, B.N., Sergienko, P.M., Titov, V.M., Tolochko, B.P., and Sheromov, M.A., Current Status of the Studies of Biological Objects by the Time-Resolved X-Ray Diffraction Technique, Nucl. Instrum. Methods Phys. Res., Sect. A, 2005, vol. 543, pp. 143–147.

    Article  ADS  Google Scholar 

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Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Sciences, Institutskaya ul. 3, Pushchino, Moscow region, 142290, Russia

    V. N. Korneev

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya ul. 3, Pushchino, Moscow region, 142290, Russia

    V. A. Shlektarev, N. F. Lanina, B. I. Medvedev & A. A. Vazina

  3. Russian Research Centre “Kurchatov Institute,”, pl. Akademika Kurchatova 1, Moscow, 123182, Russia

    A. V. Zabelin & O. V. Nayda

  4. Budker Institute of Nuclear Physics, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent’eva 11, Novosibirsk, 630090, Russia

    V. M. Aul’chenko

  5. Institute of Solid State Chemistry and Mechanochemistry, Siberian Branch, Russian Academy of Sciences, ul. Kutateladze 18, Novosibirsk, 630090, Russia

    B. P. Tolochko

Authors
  1. V. N. Korneev
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  2. V. A. Shlektarev
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  3. A. V. Zabelin
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  4. V. M. Aul’chenko
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  5. B. P. Tolochko
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  6. N. F. Lanina
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  7. B. I. Medvedev
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  8. O. V. Nayda
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  9. A. A. Vazina
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Corresponding author

Correspondence to V. N. Korneev.

Additional information

Original Russian Text © V N. Korneev, V.A. Shlektarev, A.V. Zabelin, V.M. Aul’chenko, B.P. Tolochko, N.F. Lanina, B.I. Medvedev, O.V. Nayda, A.A. Vazina, 2010, published in Fizika i Khimiya Stekla.

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Korneev, V.N., Shlektarev, V.A., Zabelin, A.V. et al. Equipment for investigations of biological nanostructures by diffraction methods using synchrotron radiation. Glass Phys Chem 36, 100–109 (2010). https://doi.org/10.1134/S1087659610010177

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  • DOI: https://doi.org/10.1134/S1087659610010177

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