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The effects of micromycete extracellular proteases of the Aspergillus genus on the proteins of the haemostatic system

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Abstract

The micromycetes of the Aspergillus genus were screened for the evaluation of their capacity to secrete extracellular proteolytic enzymes effecting proteins of the human hemostatic system. Aspergillus extracellular proteases were found to cleave chromogenic peptide substrates specific for the hemostatic proteins, as well as to activate some proenzymes (protein C, factor X, and prothrombin). The activation of human blood plasma X-factor by micromycete extracellular proteases was first shown.

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Abbreviations

PTC:

prothrombin complex

Glp:

pyroglutamic acid

Z:

N-benzyloxycarbonyl

Bz:

benzoyl

Tos:

tosyl

Pip:

piperosyl

pNA:

p-nitroanilide

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Authors and Affiliations

  1. Biological Faculty, Moscow State University, Moscow, 119234, Russia

    A. Osmolovskiy, E. S. Zvonareva, V. G. Kreyer & N. A. Baranova

  2. International Biotechnological Center, Moscow State University, Moscow, 119234, Russia

    N. S. Egorov

Authors
  1. A. Osmolovskiy
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  2. E. S. Zvonareva
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  3. V. G. Kreyer
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  4. N. A. Baranova
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  5. N. S. Egorov
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Corresponding author

Correspondence to A. Osmolovskiy.

Additional information

Original Russian Text © A. Osmolovskiy, E.S. Zvonareva, V.G. Kreyer, N.A. Baranova, N.S. Egorov, 2014, published in Bioorganicheskaya Khimiya, 2014, Vol. 40, No. 6, pp. 688–694.

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Osmolovskiy, A., Zvonareva, E.S., Kreyer, V.G. et al. The effects of micromycete extracellular proteases of the Aspergillus genus on the proteins of the haemostatic system. Russ J Bioorg Chem 40, 634–639 (2014). https://doi.org/10.1134/S1068162014060120

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  • DOI: https://doi.org/10.1134/S1068162014060120

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