Abstract
The level of proteolytic activity in tissues of oat seedlings was characterized under acidic conditions, and the number and content of the main components in low-molecular-mass fractions of the extract were determined. The structures of the majority of predominant peptide components isolated from the extract were studied. The use of a database of protein structures helped suggest possible structures of protein precursors of the peptides isolated. Detailed information on a plant peptidome was obtained for the first time.
Similar content being viewed by others
Abbreviations
- EDTA:
-
ethylenediaminetetraacetatic acid disodium salt
- PMSF:
-
phenylmethylsulfonyl fluoride
- rpHPLC:
-
reversed phase high performance liquid chromatography
References
Ivanov, V.T., Karelin, A.A., Mikhaleva, I.I., Vas’kovskii, B.V., Sviryaev, V.I., and Nazimov, I.V., Bioorg. Khim., 1992, vol. 18, pp. 1271–1311.
Karelin, A.A., Philippova, M.M., Karelina, E.V., Strizhkov, B.N., Grishina, G.A., Nazimov, I.V., and Ivanov, V.T., J. Peptide Sci., 1998, vol. 4, pp. 211–225.
Ivanov, V.T., Karelin, A.A., Philippova, M.M., Nazimov, I.V., and Pletnev, V.Z., Biopolymers, Peptide Science, 1997, vol. 43, pp. 171–188.
Blishchenko, E.Yu., Mernenko, O.A., Mirkina, I.I., Satpaev, D.K., Ivanov, V.S., Tchirkin, L.D., Ostrovsky, A.G., Karelin, A.A., and Ivanov, V.T., Peptides, 1997, vol. 18, pp. 79–85.
Franssen, H. and Bisseling, T., Proc. Natl. Acad. Sci. USA, 2001, vol. 98, pp. 12 855–12 856.
Matsubayashi, Y., J. Cell Sci., 2003, vol. 116, pp. 3863–3870.
Ivanov, V.T., Karelin, A.A., and Yatskin, O.N., Biopolymers, 2005, vol. 80, pp. 332–346.
Holley, S.R., Yalamanchili, R.D., Moura, D.S., Ryan, C.A., and Stratmann, J.W., Plant Physiol., 2003, vol. 132, pp. 1728–1738.
Ryan, C.A., Biochim. Biophys. Acta, 2000, vol. 1477, pp. 112–121.
Constabel, C.P., Yip, L., and Ryan, C.A., Plant. Mol. Biol., 1996, vol. 36, pp. 55–62.
Matsubayashi, Y., Takagi, L., and Sakagami, Y., Proc. Natl. Acad. Sci. USA, 1997, vol. 94, pp. 13 357–13 362.
Yang, H., Matsubayashi, Y., Hanai, H., and Sakagami, Y., Plant Cell Physiol., 2000, vol. 41, pp. 825–830.
Hanai, H., Matsuno, T., Yamamoto, M., Matsubayashi, Y., Kobayashi, T., Kamada, H., and Sakagami, Y., Plant Cell Physiol., 2000, vol. 41, pp. 27–32.
Clark, S.E., Running, M.P., and Meyerowitz, E.M., Development, 1993, vol. 119, pp. 397–418.
Fletcher, J.C., Brand, U., Running, M.P., Simon, R., and Meyerowitz, E.M., Science, 1999, vol. 283, pp. 1911–1914.
Karelin, A.A., Philippova, M.M., Yatskin, O.N., Kalinina, O.A., Nazimov, I.V., Blishchenko, E.Yu., and Ivanov, V.T., J. Peptide Sci., 2000, vol. 6, pp. 345–354.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.G. Gara, O.N. Yatskin, V.I. Shvets, A.A. Karelin, V.T. Ivanov, 2006, published in Bioorganicheskaya Khimiya, 2006, Vol. 32, No. 2, pp. 211–220.
Rights and permissions
About this article
Cite this article
Gara, O.G., Yatskin, O.N., Shvets, V.I. et al. Isolation and elucidation of structure of peptides from oat (Avena sativa) seedlings. Russ J Bioorg Chem 32, 191–200 (2006). https://doi.org/10.1134/S1068162006020129
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1134/S1068162006020129