Abstract
The content and composition of the amino acids in typical chernozems were studied. The objects of the study included a reference soil under an old fallow and three variants under fodder crop rotations: not fertilized, with mineral fertilizers, and with organic fertilizers. The contents of 18 amino acids were determined in these soils. The amino acids were extracted by the method of acid hydrolysis and identified by the method of ion-exchange chromatography. The total content of most of the amino acids was maximal in the reference soil; it was much lower in the cultivated soils and decreased in the following sequence: organic background > mineral background > no fertilization. The diversity of amino acids was evaluated quantitatively using different parameters applied in ecology for estimating various aspects of the species composition of communities (Simpson, Margalef, Menhinick, and Shannon’s indices). The diversity and contribution of different amino acids to the total pool of amino acids also varied significantly in the studied variants. The maximum diversity of amino acids and maximum evenness of their relative abundance indices were typical of the reference chernozem; these parameters were lower in the cultivated soils. It was concluded that the changes in the structure of the amino acids under the impact of agricultural loads are similar to those that are usually observed under stress conditions.
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References
B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J. D. Watson, Molecular Biology of the Cell, 2nd ed. (Garland Publ., NY, 1989).
A. I. Bakanov, “Quantitative Assessment of Dominance in Ecological Communities,” VINITI, No. 8593, 12 (1987).
A. L. Efremov, “The content of free amino acids in soils under the pine forests of Belarus and Poland,” Eur. Soil Sci. 33(12), 1303–1308 (2000).
D. I. Iudin, D. B. Gelashvili, and G. S. Rozenberg, “Multifractal analysis of the species structure of biotic communities,” Dokl. Biol. Sci. 389, 143–146 (2003).
T. D. Kozarenko, S. N. Zuev, and N. F. Mulyar, Ion-Exchange Chromatography of Amino Acids (Nauka, Novosibirsk, 1981) [in Russian].
B. B. Mandelbrot, The Fractal Geometry of Nature (W.G. Freeman and Co., San Francisco, 1982).
R. Margalef, Our Biosphere (Ecol. Inst., Oldendorf/Luhe, 1997).
A. E. Magurran, Ecological Diversity and Its Measurement (Princeton Univ. Press, Princeton, 1988)..
E. P. Odum, Fundamentals of Ecology, Philadelphia: Saunders, 1971.
A. Svirskene, “Microbiological and biochemical indicators of anthropogenic impacts on soils,” Eur. Soil Sci. 36(2), 192–200 (2003).
R. L. Tate, Soil Organic Matter. Biological and Ecological Effects (Wiley, Chichester, 1987).
R. H. Whittaker, Communities and Ecosystems (Macmillan, 1975).
M. M. Umarov and I. V. Aseeva, “Free amino acids in some soils of the Soviet Union,” Pochvovedenie, No. 10, 38–52 (1971).
M. M. Umarov, A. V. Kurakov, and A. L. Stepanov, Microbiological Transformation of Nitrogen in Soil (Geos, Moscow, 2008) [in Russian].
N. I. Frunze, “Amino acid pool of a typical chernozem of Moldova,” Eur. Soil Sci. 44(10), 1139–1143 (2011).
I. I. Dediu, Enciclopedie de Ecologie (Stiinta, Chisinau, 2010).
A. L. Leninger, Biochimie, Vol. 2 (Editura Tehnica, Bucuresti, 1995).
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Original Russian Text © N.I. Frunze, 2014, published in Pochvovedenie, 2014, No. 12, pp. 1483–1489.
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Frunze, N.I. Diversity of amino acids in a typical chernozem of Moldova. Eurasian Soil Sc. 47, 1259–1265 (2014). https://doi.org/10.1134/S1064229314120035
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DOI: https://doi.org/10.1134/S1064229314120035