Abstract
By means of dye L7012, the number and physiological condition (damage rate of membranes) of bacterial cells is defined. The results testify to considerable physiological heterogeneity of bacteria cells in soils. In fresh samples of soil, the percentage of intact cells reached 60–70%. Damaged membranes occurred in 30–40% of cells. The number of damaged cells dramatically increased downwards through the soil profile. Drying and freezing of soil samples considerably reduced the quantity of intact cells and increased the percentage of cells with damaged membranes; the number of intact cells was 10–20%. Treatment with biocide agents resulted in lysis of the majority of cells and cells injuries. However, some of the cells kept an intact cellular membrane, which testifies to the high stability of bacteria in soil. These data allow us to offer a method of staining a soil suspension with the use of luminescent dye L7012 with the quality of an express method that gives the chance to monitor the number and physiological condition of the bacterial complex of soils.
This is a preview of subscription content, log in via an institution to check access.
References
Auty, M.A.E., Gardiner, G.E., McBrearty, S.J., et al., Direct in Situ Viability Assessment of Bacteria in Probiotic Dairy Products Using Viability Straining in Conjunction with Confocal Scanning Laser Microscopy, Appl. Environ. Microbiol., 2001, vol. 67, no. 1, pp. 420–425.
Gray, T.R.G, in Methods in microbiology, vol. 22, Grigorova, R. and Norris, J.R.N.Y., Eds.,. Acad. Press, 1990. pp. 309–342.
LIVE / DEAD BacLight Bacterial Viability Kit Instruction Manual with Appendix, Molecular Probes, 1994.
Lapygina, E.V., Lysak, L.V., Bakulina, E.A., and Zvyagintsev, D.G., Resistance of Autochthonous Soil Bacteria to Sock Biocide Impacts, Pochvovedenie, 2006, no. 11, pp. 1363–1368.
Metody pochvennoi biokhimii i mikrobiologii (Methohds of Soil Biochemistry and Microbiology), Zvyagintsev, D.G., Ed., Moscow: Mosk. Gos. Univ., 1991.
Polyanskaya, L.M., Geidebrekht, V.V., Stepanov, A.L., and Zvyagintsev, D.G., Distribution of Abundance and Biomass of Microorganisms along the Profile of Zonal Soil Types, Pochvovedenie, 1995, no. 3, pp. 322–328.
Polyanskii, A.M., Golovchenko, A.V., Polyanskaya, L.M., and Zvyagintsev, D.G., New Criteria for the Evaluation of Soil Bacterial Complexes, Mikrobiologiya, 2002, vol. 71, no. 5, pp. 675–680.
Terzieva, S., Donnelly, J., Ulevicius, V., et al., Comparison of Methods for Detection and Enumeration of Airborne Microorganisms Collected by Liquid Impingement, Appl. Environ. Microbiol., 1996, vol. 62, no. 7, pp. 2264–2272.
Zvyagintsev, D.G., Pochvy i mikroorganizmy (Soils and Microorganisms), Moscow: Mosk. Gos. Univ., 1987.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © L.V. Lysak, E.V. Lapygina, I.A. Konova, D.G. Zvyagintsev, 2009, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2009, No. 6, pp. 750–754.
Rights and permissions
About this article
Cite this article
Lysak, L.V., Lapygina, E.V., Konova, I.A. et al. Definition of the physiological condition of bacteria in soil by means of luminescent dye L7012. Biol Bull Russ Acad Sci 36, 639–642 (2009). https://doi.org/10.1134/S1062359009060156
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1062359009060156