Abstract
The effects of prostaglandin PGE2 on apoptosis and antioxidant enzyme activities were studied in two coelomocyte fractions of holothurian Eupentacta fraudatrix in vitro and in vivo. PGE2 (10−8–10−6M) modulated apoptosis in a time-and concentration-dependent manner in both fractions studied in vitro. In vivo, PGE2 induced apoptosis at concentrations of 0.1–1 μg/g in the fraction enriched with morula-like cells. Phagocytes were more sensitive to the regulating effect of PGE2. In this fraction, PGE2 induced apoptosis at concentrations from 0.01 to 1 μg/g, while PGE2 at 10 μg/g demonstrated an antiapoptotic effect. In all experiments, apoptosis development was accompanied by a disbalance of the antioxidant enzyme system, primarily, decreased catalase activity.
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Original Russian Text © L.S. Dolmatova, O.A. Zaika, 2007, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2007, No. 3, pp. 273–282.
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Dolmatova, L.S., Zaika, O.A. Apoptosis-modulating effect of prostaglandin E2 in coelomocytes of holothurian Eupentacta fraudatrix depends on the cell antioxidant enzyme status. Biol Bull Russ Acad Sci 34, 221–229 (2007). https://doi.org/10.1134/S1062359007030028
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DOI: https://doi.org/10.1134/S1062359007030028