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Development and Validation of Analytical Method for Simultaneous Estimation of Tazarotene and Hydroquinone in their Gel Formulation by Reversed-phase High Performance Liquid Chromatography

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Abstract

A simple, sensitive, rapid, precise and accurate method has been developed for simultaneous estimation of tazarotene and hydroquinone in their pharmaceutical dosage form (gel preparation). The chromatographic separation was carried out on a reversed-phase Inertsil C18 (4.6 mm I.D. × 250 mm, 5 μm) column using a mobile phase consisting of 0.02 M KH2PO4‒acetonitrile (80 : 20, v/v) at a flow rate of 1.0 mL/min and UV detection at λmax = 254 nm. The method showed linearity with the correlation coefficient of 0.999 for both tazarotene and hydroquinone over the concentration range of 5‒25 and 1‒5 µg/mL, respectively. The mean recoveries were found to be in the range of 98‒101% for both components. The method was validated as per International Conference on Harmonization guidelines for linearity, limit of detection, limit of quantification, specificity, accuracy, precision and robustness. As a result, this method can be successfully applied for routine quantification of tazarotene and hydroquinone in pharmaceutical dosage form (gel preparation).

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ACKNOWLEDGMENTS

Authors gratefully acknowledge Vice-chancellor, Banasthali Vidyapith for providing necessary research facilities. The financial assistance provided by the DST, New Delhi under the CURIE program is duly acknowledged.

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Authors and Affiliations

  1. Department of Pharmacy, Banasthali Vidyapith, Rajasthan, Banasthali-304022, India

    Yadav Rajkumar, Yadav Divya & Yadav Rakesh

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  1. Yadav Rajkumar
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  2. Yadav Divya
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  3. Yadav Rakesh
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Correspondence to Yadav Rakesh.

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Yadav Rajkumar, Divya, Y. & Rakesh, Y. Development and Validation of Analytical Method for Simultaneous Estimation of Tazarotene and Hydroquinone in their Gel Formulation by Reversed-phase High Performance Liquid Chromatography. J Anal Chem 74, 467–471 (2019). https://doi.org/10.1134/S1061934819050095

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  • DOI: https://doi.org/10.1134/S1061934819050095

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