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Simultaneous determination of residual amounts of amphenicols in food by HPLC with UV-detection

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Abstract

A rapid, sensitive, and safe method has been proposed for the simultaneous determination of residual amounts of chloramphenicol, thiamphenicol, and chloramphenicol in food by high-performance liquid chromatography with ultraviolet detection at 228 and 280 nm using a combination of QuEChERS and dispersive liquid-liquid microextraction (DLLME). The optimum conditions of QuEChERS and DLLME sample preparation ensuring sufficient preconcentration and additional purification of the extract have been found. Recovery was 64–87% depending on the sample matrix nature. Under the selected optimum conditions, the analytical ranges taking into account the concentration coefficient equal to 10 were 0.01–5 mg/kg. The relative standard deviation of the results of analysis did not exceed 9%. The time of analysis was 1–1.5 h.

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Authors and Affiliations

  1. Federal Center for Animals Health, Yur’evets, Vladimir, 600901, Russia

    V. G. Amelin, N. M. Volkova, N. A. Repin & T. B. Nikeshina

  2. Vladimir State University, ul. Gor’kogo 87, Vladimir, 600000, Russia

    V. G. Amelin, N. M. Volkova & N. A. Repin

Authors
  1. V. G. Amelin
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  2. N. M. Volkova
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  3. N. A. Repin
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  4. T. B. Nikeshina
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Correspondence to V. G. Amelin.

Additional information

Original Russian Text © V.G. Amelin, N.M. Volkova, N.A. Repin, T.B. Nikeshina, 2015, published in Zhurnal Analiticheskoi Khimii, 2015, Vol. 70, No. 10, pp. 1114–1120.

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Amelin, V.G., Volkova, N.M., Repin, N.A. et al. Simultaneous determination of residual amounts of amphenicols in food by HPLC with UV-detection. J Anal Chem 70, 1282–1287 (2015). https://doi.org/10.1134/S1061934815100020

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  • DOI: https://doi.org/10.1134/S1061934815100020

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