Skip to main content
SpringerLink
Log in

Generation of characteristic profiles of steroid hormones by reversed-phase HPLC

  • Articles
  • Published:
Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

A method for the determination of steroid hormones by reversed-phase HPLC has been applied to the analysis of biological fluids from healthy individuals and patients with endocrine systems diseases. The obtained chromatographic profiles were processed by the method of principal component analysis and method of soft independent modeling of class analogy. The principle possibility of using the results of chemometric processing of the chromatographic profiles as an additional diagnostic criterion for the treatment of some endocrine pathologies has been shown.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kartsova, L.A. and Obedkova, E.V., J. Anal. Chem., 2013, vol. 68, no. 4, p. 291.

    Article  CAS  Google Scholar 

  2. Orlov, E.N., Antipov, E.M., and Nikolaev, N.N., Vopr. Med. Khim., 1993, no. 5, p. 7.

    Google Scholar 

  3. Orlov, E.N., Nikolaev, N.N., Antipov, E.M., Chmozh L.A., and Makarov, O.V., Probl. Endokrinol., 1995, vol. 41, no. 2, p. 35.

    CAS  Google Scholar 

  4. Shakelton, C.H.L., J. Chromatogr. Biochem. Appl., 1986, vol. 379, p. 91.

    Article  Google Scholar 

  5. Rossi, C., Caiton, L., Hammond, G., Brown, H.A., Wallace, M., Sacchetta, P., and Morris, M., Clin. Chim. Acta, 2010, vol. 411, p. 222.

    Article  CAS  Google Scholar 

  6. Cho, H.-J., Kim, J.D., Lee, W.-Y., Chung, B.C., and Choi, M.H., Clin. Chim. Acta, 2009, vol. 632, p. 101.

    Article  CAS  Google Scholar 

  7. Arlt, W., Biehl, M., Taylor, A.E., Hahner, S., Libe, R., Hughes, B.A., Schneider, P., Smith, D.J., Stiekema, H., Krone, N., Porfiri, E., Opocher, G., Bertherat, J., Mantero, F., Allolio, B., Terzolo, M., Nightingale, P., Shackleton, C.H.L., Bertagna, X., Fassnacht, M., and Stewart, P.M., J. Clin. Endocrinol. Metab., 2011, vol. 96, p. 3775.

    Article  CAS  Google Scholar 

  8. Duarte, A.C. and Capelo, S., J. Liq. Chromatogr. and Relat. Technol., 2006, vol. 29, p. 1143.

    Article  CAS  Google Scholar 

  9. Daszykowski, M. and Walczak, B., Trends Anal. Chem. Pers. Ed., 2006, vol. 25, p. 1081.

    Article  CAS  Google Scholar 

  10. Esbensen, K.H., Multivariate Data Analysis in Practice. An Introduction to Multivariate Data Analysis and Experimental Design, Oslo: Camo Software AS, 2010, 5th Ed.

    Google Scholar 

  11. Beebe, K.R., Pell, R.J., and Seasholtz, M.B., Chemometrics: a Practical Guide, New York: Wiley.

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, St. Petersburg State University, Universitetskii pr. 26, Staryi Petergof, St. Petersburg, 198504, Russia

    E. V. Obedkova, L. A. Kartsova, D. O. Kirsanov & A. V. Legin

  2. Mechnikov North-Western State Medical University, ul. Kirochnaya 41, St. Petersburg, 191015, Russia

    L. I. Velikanova

Authors
  1. E. V. Obedkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. A. Kartsova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. O. Kirsanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. I. Velikanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. V. Legin
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Original Russian Text © E.V. Obedkova, L.A. Kartsova, D.O. Kirsanov, L.I. Velikanova, A.V. Legin, 2014, published in Zhurnal Analiticheskoi Khimii, 2014, Vol. 69, No. 2, pp. 214–218.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Obedkova, E.V., Kartsova, L.A., Kirsanov, D.O. et al. Generation of characteristic profiles of steroid hormones by reversed-phase HPLC. J Anal Chem 69, 200–204 (2014). https://doi.org/10.1134/S1061934814020117

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1061934814020117

Keywords

Search

Navigation