Abstract
The advantages and potencies of direct methods for the quantification of homocysteine based on mass spectrometry detection (HPLC-MS and CE-MS) are considered; these significantly simplify sample preparation and exclude derivatization with expensive derivatizing reagents. An approach is developed for the direct determination of total homocysteine in blood plasma in an analytical system that combines capillary electrophoresis with electrospray ionization. The internal standard is glycyl-asparagine; the duration of separation is 25 min. The sample is preconcentrated tenfold prior to the determination. The sample pretreatment also includes the precipitation of proteins with methanol and the reduction of amino thiols with dithiothreitol or mercaptoethanol. The recovery values make 87–112%.
Similar content being viewed by others
References
Svardal, A., Refsum, H., and Ueland, P.M., JBC, 1986, vol. 261, no. 7, p. 3156.
Refsum, H., Helland, S., and Uerland, P.M., Clin. Chem., 1985, vol. 31, p. 624.
Togawa, T., Sengupta, S., Chen, H., Robinson, K., Nonevski, I., Majors, A.K., and Jacobsen, D.W., Biochem. Biophys. Res. Commun., 2000, vol. 277, p. 668.
Mills, B.J., Weiss, M.M., Lang, C.A., Liu, M.C., and Ziegler, C., J. Lab. Clin. Med., 2000, vol. 135, p. 396.
Fermo, I., Vecchi, E., Arcelloni, C., D’Angelo, A., and Paroni, R., Haematologica, 1997, vol. 82, p. 246.
Lim, A., Sengupta, S., McComb, M.E., Theberge, R., Wilson, W.G., Costello, C.E., and Jacobsen, D.W., JBC, 2003, vol. 278, no. 50, p. 49707.
Brustolin, S., Giugliani, R., and Flix, T.M., Braz. J. Med. Biol. Res. 2010, vol. 43, no. 1, p. 1.
Veldman, B.A., Verwoort, G., Blom, H., and Smits, P., Diab. Med., 2005, vol. 22, p. 301.
Araki, A. and Sako, Y., J. Chromatogr., 1987, vol. 422, p. 43.
Refsum, H., Ueland, P.M., and Svardal, A.M., Clin. Chem., 1989, vol. 35, no. 9, p. 1921.
Fermo, I., Arcelloni, C., De Vecchi, E., Vigan, S., and Paroni, R., J. Chromatogr., 1992, vol. 593, nos. 1–2, p. 171.
Hyland, K. and Bottiglieri, T., J. Chromatogr., 1992, vol. 579, no. 1, p. 55.
Nolin, T.D., McMenamin, M.E., and Himmelfarb, J., J. Chromatogr. B, 2007, vol. 852, nos. 1–2, p. 554.
Kang, S.H., Wei, W., and Yeung, E.S., J. Chromatogr. B, 2000, vol. 744, p. 149.
Mel’nikov, I.O., Nazimov, I.V., Stukacheva, E.A., and Glubokov, Yu.M., Zhurn. anal. khim., 2006, vol. 61, no. 11, p. 1185 [J. Anal. Chem. (Engl. Transl.), vol. 61, no. 11, p. 1093].
Kartsova, L.A., Sidorova, A.A., and Ivanova, A.S., Zhurn. anal. khim., 2007, vol. 62, no. 10, p. 1066 [J. Anal. Chem. (Engl. Transl.), vol. 62, no. 10, p. 960].
Stamler, J.S., Nutr. Rev., 1996, vol. 54, p. 1.
Malloy, M.H., et al., Anal. Biochem., 1981, vol. 113, p. 407.
Iciek, M., Chwatko, G., Lorenc-Koci, E., Bald, E., and Wodek, L., Acta Biochim. Pol., 2004, vol. 51, no. 3, p. 815.
Malinow, M.R., Kang, S.S., Taylor, L.M., Wong, P.W., Coull, B., Inahara, T., Mukerjee, D., Sexton, G., and Upson, B., Circulation, 1989, vol. 79, no. 6, p. 1180.
Accinni, R., Bartesaghi, S., De Leo, G., Cursano, C.F., Achilli, G., Loaldi, A., Cellerino, C., and Parodi, O., J. Chromatogr. A, 2000, vol. 896, nos. 1–2, p. 183.
Shipchandler, M.T. and Moore, E.G., Clin. Chem., 1995, vol. 41, no. 7, p. 991.
Powers, H.J. and Moat, S.J., Curr. Opin. Clin. Nutrition Metabolic Care, 2000, vol. 3, no. 5, p. 391.
Stabler, S., Marcell, P., Podell, E., and Allen, R., Anal. Biochem., 1987, vol. 162, p. 185.
Valerio, A., Baldo, G., and Tessari, P., Rapid Commun. Mass. Spectrom., 2005, vol. 19, no. 4, p. 561.
Jiang, Z., Liang, Q., Luo, G., Hu, P., Li, P., and Wang, Y., Talanta, 2009, vol. 77, p. 1279.
Li, S., Jia, J., Liu, G., Wang, W., Cai, Y., Wang, Y., and Yu, C., J. Chromatogr. B, 2008, vol. 870, p. 63.
Rafii, M., Elango, R., House, J.D., Courtney-Martin, G., Darling, P., Fisher, L., and Pencharz, P.B., J. Chromatogr. B, 2009, vol. 877, p. 3282.
Matuszewski, B.K., Constanzer, M.L., and Chavez-Eng, C.M., Anal. Chem., 2003, vol. 75, p. 3019.
Gellekink, H., van Oppenraaij-Emmerzaal, D., van Rooij, A., Struys, E.A., Heijer, M., and Blom, H.J., Clin. Chem., 2005, vol. 51, no. 8, p. 1487.
Moini, M., Anal. Bioanal. Chem., 2002, vol. 373, p. 466.
Ohnesorge, J., Neususs, C., and Watzig, H., Electrophoresis, 2005, vol. 26, no. 21, p. 3973.
Chwatko, G. and Jakubowski, H., Anal. Biochem., 2005, vol. 337, no. 2, p. 271.
Author information
Authors and Affiliations
Additional information
Original Russian Text © A.V. Ivanov, B.P. Luzyanin, A.A. Moskovtsev, A.S. Rotkina, A.A. Kubatiev, 2011, published in Zhurnal Analiticheskoi Khimii, 2011, Vol. 66, No. 3, pp. 324–328.
Rights and permissions
About this article
Cite this article
Ivanov, A.V., Luzyanin, B.P., Moskovtsev, A.A. et al. Determination of total homocysteine in blood plasma by capillary electrophoresis with mass spectrometry detection. J Anal Chem 66, 317–321 (2011). https://doi.org/10.1134/S1061934811030075
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934811030075