Abstract
Microelectrophoresis, dynamic light scattering, fluorescence, and microcalorimetry are used to study the adsorption of a synthetic polycation, poly-N-ethyl-4-vinylpyridinium bromide, on the surface of three-component liposomes formed from electrically neutral phosphatidylcholine, anionic diphosphatidylglycerol (cardiolipin), and cationic dicetyldimethylammonium bromide, with the two latter being taken in equal amounts. The adsorption of the polycation on the liposomal membrane results in the generation of a positive charge, which provides the polycation-liposome complex with aggregation stability. Increasing salt concentration in the suspension causes the complex to dissociate into its components. According to the microcalorimetry data, the membranes of the initial three-component liposomes consist of two microphases, with one of them being enriched with the neutral lipid and another one, with the ionic components. The polycation adsorption does not lead to noticeable structural rearrangements in the liposomal membranes.
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Original Russian Text © D.A. Davydov, A.V. Romanyuk, A.A. Rakhnyanskaya, P.I. Semenyuk, V.N. Orlov, V.V. Samoshin, A.A. Yaroslavov, 2011, published in Kolloidnyi Zhurnal, 2011, Vol. 73, No. 1, pp. 36–42.
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Davydov, D.A., Romanyuk, A.V., Rakhnyanskaya, A.A. et al. Cationic polymer adsorption on bilayer membrane containing anionic and cationic lipids. Colloid J 73, 33–38 (2011). https://doi.org/10.1134/S1061933X11010042
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DOI: https://doi.org/10.1134/S1061933X11010042