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Characteristics of alpha-amylase isozymes in cytologenetically different wheat cultivars

  • Plant Genetics
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Abstract

The isoenzyme composition of alpha-amylase is studied by polyacrylamide gel electrophoresis in Tris-glycine (pH 8.3) system in wheat cultivars with different genome composition. We show that durum wheat (Triticum durum, 2n = 4x = 28, BBAA) lacks the isoenzymes encoded by 6D and 7D chromosomes that are present in common wheat zymograms (Triticum aestivum, 2n = 6x = 42, BBAADD). A similar pattern is observed in a synthetic allohexaploid carrying the BBAA genomes of wheat and the H ch H ch genome of barley (Hordeum chilense). Our method of electrophoresis fails to reveal additional variants of alpha-amylase encoded by the barley genome, although C-banding analysis confirms the genomic structure BBAAH ch H ch of this allopolyploid. The electrophoretic spectrum of the spring common wheat cultivar Dobrynya with the wheat-Agropyron translocation 7DL-7AiL contains all of the alpha-amylase isoenzymes typical for common wheat (2n = 6x = 42, BBAADD) except for the zymotype encoded by the long arm of chromosome 7D. This observation confirms the results of cytogenetic analysis that identified a 7DL-7AiL translocation in this cultivar. No additional alpha-amylase isoenzymes encoded by Agropyron chromosome have been observed. Our data indicate that analysis of wheat-alien hybrids or introgressive forms should be carried out using a complex of different methods.

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Authors and Affiliations

  1. Belgorod State Research Institute of Agriculture, Russian Academy of Agricultural Sciences, Belgorod, 308001, Russia

    V. P. Netsvetaev

  2. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    E. D. Badaeva

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  1. V. P. Netsvetaev
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  2. E. D. Badaeva
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Correspondence to V. P. Netsvetaev.

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Original Russian Text © V.P. Netsvetaev, E.D. Badaeva, 2014, published in Genetika, 2014, Vol. 50, No. 7, pp. 825–830.

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Netsvetaev, V.P., Badaeva, E.D. Characteristics of alpha-amylase isozymes in cytologenetically different wheat cultivars. Russ J Genet 50, 722–727 (2014). https://doi.org/10.1134/S102279541407014X

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  • DOI: https://doi.org/10.1134/S102279541407014X

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