Skip to main content
SpringerLink
Log in

AFLP marking of the genotypes of leek (Allium porrum) varieties

  • Short Communications
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

The results of the AFLP analysis of 16 leek (Allium porrum) accessions and related species of the sections of the genus Allium are presented. Restriction enzymes and primer combinations for the identification of the genotypes of the A. porrum accessions were chosen. As a result, 265 polymorphic AFLP fragments were amplified for 25 analyzed genotypes, and specific spectra of DNA fragments were obtained for each accession. A total of 24 fragments specific for the A. porrum genome was detected, of which only two characterized the genotypes of individual accessions. A wide range of genetic diversity (0.11–0.32) was revealed for the A. porrum varieties and lines used in the analysis. The highest level of similarity in the analyzed set of accessions was found between A. porrum and sand leek (A. scorodoprasum).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Dubouzet, J.G. and Shinoda, K., Relationships among Old and New World Alliums According to ITS DNA Sequence Analysis, Theor. Appl. Genet., 1999, vol. 98, pp. 422–433.

    Article  CAS  Google Scholar 

  2. Galeone, C., Pelucchi, C., Levi, F., et al., Onion and Garlic Use and Human Cancer, Amer. J. Clin. Nutrition, 2006, vol. 84, no. 5, pp. 1027–1032.

    CAS  Google Scholar 

  3. Smilde, W.D., van Heusden, A.W., and Kik, C., AFLPs in Leek (Allium porrum) Are not Inherited in Large Linkage Blocks, Euphytica, 1999, vol. 110, pp. 127–132.

    Article  CAS  Google Scholar 

  4. Toquica, S.P., Rodriguez, F., Martinez, E., et al., Molecular Characterization by AFLPs of Capsicum germplasm from the Amazon Department in Colombia, Characterization by AFLPs of Capsicum, Genet. Resour. Crop Evol., 2003, vol. 50, pp. 639–647.

    Article  Google Scholar 

  5. Fleischer, R., Horlemann, C., Schwekendiek, A., et al., AFLP Fingerprinting in Hop: Analysis of the Genetic Variability of the Tettnang Variety, Genet. Resour. Crop Evol., 2004, vol. 51, no. 2, pp. 211–220.

    Article  Google Scholar 

  6. Blaich, R., Konradi, J., Rühl, E., and Forneck, A., Assessing Genetic Variation among Pinot Noir (Vitis vinifera L.) clones with AFLP Markers, Am. J. Enol. Vitic., 2007, vol. 58, no. 4, pp. 526–529.

    CAS  Google Scholar 

  7. Sargent, D.J., Fernández-Fernández, F., Rys, A., et al., Mapping of A1 Conferring Resistance to the Aphid Amphorophora idaei and DW (Dwarfing Habit) in Red Raspberry (Rubus idaeus L.) Using AFLP and Microsatellite Markers, BMC Plant Biol., 2007, vol. 7, no. 15, pp. 1–9.

    Google Scholar 

  8. Kim, G.H., Yun, H.K., Choi, C.S., et al., Identification of AFLP and RAPD Markers Linked to Anthracnose Resistance in Grapes and Their Conversion to SCAR Markers, Plant Breed., 2008, vol. 127, no. 4, pp. 418–423.

    Article  CAS  Google Scholar 

  9. Burger J.C., Chapman M.A., and Burke, J.M., Molecular Insights into the Evolution of Crop Plants, Am. J. Bot., 2008, vol. 95, pp. 113–122.

    Article  Google Scholar 

  10. Edwards, S.K., Johonstone, C., and Thompson, C., Simple and Rapid Method for the Preparation of Plant Genomic DNA for PCR Analysis, Nucleic Acids Res., 1991, vol. 19, no. 6, p. 1349.

    Article  PubMed  CAS  Google Scholar 

  11. Vos, P., Hogers, R., Bleeker, M., et al., AFLP: A New Technique for DNA Fingerprinting, Nucleic Acids Res., 1995, vol. 23, pp. 4407–4414.

    Article  PubMed  CAS  Google Scholar 

  12. Van de Peer, Y. and Wachter, R., TREECON for Windows: A Software Package for the Construction and Drawing of Evolutionary Trees for the Microsoft Windows Environment, Comput. Appl. Biosci, 1994, vol. 10, pp. 569–570.

    PubMed  Google Scholar 

  13. Heusden, A.W., Shigyo, M., Tashiro, Y., et al., AFLP Linkage Group Assignment to the Chromosomes of Allium cepa L. via Monosomic Addition Lines, Theor. Appl. Genet., 2000, vol. 100, pp. 480–486.

    Article  Google Scholar 

  14. Ipek, M., Ipek, A., and Simon, P.W., Genetic Characterization of Allium tuncelianum: An Endemic Edible Allium Species with Garlic Odor, Scientia Horticulturae, 2008, vol. 115, pp. 409–415.

    Article  CAS  Google Scholar 

  15. Friesen, N., Pollner, S., Bachmann, K., and Blattner, F.R., RAPDs and Noncoding Chloroplast DNA Reveal a Single Origin of the Cultivated Allium fistulosum from A. altaicum (Alliaceae), Am. J. Bot., 1999, vol. 86, no. 4, pp. 554–562.

    Article  PubMed  CAS  Google Scholar 

  16. Volk, G.M., Henk, A.D., and Richards, C.M., Genetic Diversity among US Garlic Clones as Detected Using AFLP Methods, J. Amer. Soc. Hort. Sci., 2004, vol. 129, no. 4, pp. 559–569.

    CAS  Google Scholar 

  17. Ovesna, J., Kuchera, L., Kralova, J., et al., Genetic Diversity among Garlic Clones as Revealed by AFLP, Phenotypic Descriptors and S-Amino Acidic Level, Vegetative Crops Res. Bull., 2007, vol. 66, pp. 105–116.

    Article  Google Scholar 

  18. Raamsdonk, L.W.D., Vrielink-van Ginkel, M., and Kik, C., Phylogeny Reconstruction and Hybrid Analysis in Allium Subgenus Rhizirideum, Theor. Appl. Genet., 2000, vol. 100, pp. 1000–1009.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bioengineering Center, Russian Academy of Sciences, Moscow, 117312, Russia

    M. A. Filjushin, O. A. Kholda, E. Z. Kochieva & N. N. Ryzhova

Authors
  1. M. A. Filjushin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. A. Kholda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Z. Kochieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. N. Ryzhova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. Z. Kochieva.

Additional information

Original Russian Text © M.A. Filjushin, O.A. Kholda, E.Z. Kochieva, N.N. Ryzhova, 2011, published in Genetika, 2011, Vol. 47, No. 4, pp. 560–565.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Filjushin, M.A., Kholda, O.A., Kochieva, E.Z. et al. AFLP marking of the genotypes of leek (Allium porrum) varieties. Russ J Genet 47, 492–496 (2011). https://doi.org/10.1134/S1022795411030045

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795411030045

Keywords

Search

Navigation