Abstract
Polymorphism analysis of DNA fragments flanked by (AG)9C and (GA)9C inverted dinucleotide microsatellite repeats in 766 animals of 19 cattle breeds and one breeding type revealed 66 fragments, of which 64 were polymorphic. The breeds proved to differ in the frequency and presence or absence of amplified DNA fragments at the genomic level, indicating that ISSR fingerprinting is informative for differentiating the PCR product spectra and cattle breeds. Multilocus ISSR polymorphism analysis identified the group of fragments that can be used as Bos taurus and B. indicus species markers to describe the standards of breeds, their genetic profiles, and breed-specific patterns. Based on ISSR polymorphism, a prototypal gene pool of cattle was constructed and the breeds closest to it were identified. Genetic diversity analysis made it possible to assume that an optimal mean heterozygosity is characteristic of cattle breeds and that deviations from this optimum are indicative of various processes occurring in the population (breed).
Similar content being viewed by others
References
Jeffreys, A.J., Wilson, V., and Thein, S.L., Individual-Specific “Fingerprints” of Human DNA, Nature, 1985, vol. 316, pp. 76–79.
Sulimova, G.E., DNA-Markers in Genetic Studies: Types of Markers, Their Properties and the Range of Application, Usp. Sovrem. Biol., 2004, vol. 124, no. 3, pp. 260–271.
Kharchenko, P.N. and Glazko, V.I., DNK-tekhnologii v razvitii agrobiologii (DNA Technologies in Development of Agrobiology), Vanyushin, B.F., Ed., Moscow, 2006.
Zietkiewicz, E., Rafalski, A., and Labuda, D., Genome Fingerprinting by Sequence Repeat (SSR)-Anchored Polymerase Chain Reaction Amplification, Genomics, 1994, pp. 176–183.
STATISTICA for Windows (Computer Program Manual), Tulsa: StatSoft, 1996.
Yeh, F.C., Yang, R.C., and Boyle, T., POPGENE. Microsoft Windows-Based Freeware for Population Genetic Analysis: Release 1.31, Edmonton: Univ. Alberta, 1999.
Nei, M., Genetic Distance between Populations, Am. Naturalist, 1972, no. 949, pp. 283–292.
Nei, M., Molecular Evolutionary Genetics, New York: Columbia University Press, 1987, pp. 176–187.
Nei, M. and Kumar, S., Molecular Evolution and Phylogenetics, Oxford: Oxford University Press, 2000.
Stolpovskii, Yu.A., Kol, N.V., Evsyukov, A.N., et al., Analysis of Population Genetic Structure of Tuvinian Short-Fat-Tail Sheep Using ISSR-PCR Method, Russ. J. Genet., 2010, vol. 46, no. 12, pp. 1660–1669.
Altukhov, Yu.P. and Rychkov, Yu.G., Population Systems and Their Structural Components: Genetic Stability and Variation, Zh. Obshch. Biol., 1970, vol. 31, no. 5, pp. 507–526.
Rychkov, Yu.G., System of Ancient Human Isolates in Northern Asia from the Genetic Aspects of Evolution and Stability of Populations, Vopr. Antropol., 1973, no. 44, pp. 3–22.
Krasota, V.F., Lobanov, V.T., and Dzhaparidze, T.G., Razvedenie sel’skokhozyaistvennykh zhivotnykh (Livestock Breeding), Moscow: Kolos, 1983.
Loftus, R.T., MacHugh, D.E., Bradley, D.G., et al., Evidence for Two Independent Domestications of Cattle, Proc. Natl. Acad. Sci. USA, 1994, vol. 91, pp. 2757–2761.
Kolesnik, N.N., Evolyutsiya krupnogo rogatogo skota (Evolution of Cattle), Stalinabad: Tadzh. Fil. Akad. Nauk SSSR, 1949.
Kozir’, V.S., Barabash, V.I., Oliinik, S.O., et al., The Ukrainian Grey Cattle: Past, Present, and Future, Kozirya, V.S., Ed., Dnipropetrovs’k.: Ukrains’ka Akad. Agrarnikh Nauk, Institut tvarinnitstva tsentral’nikh raioniv, 2008.
Livshits, G. and Kobylansky, E., Lerner’s Concept of Development Homeostasis and the Problem of Heterozygosity Level in Natural Populations, Heredity, 1985, vol. 55, pp. 341–353.
Leary, R.F., Allendorf, F.W., and Knudsen, K.L., Differences in Inbreeding Coefficients Do not Explain the Association between Heterozygosity at Allozyme Loci and Developmental Stability in Rainbow Trout, Evolution, 1987, vol. 41, no. 6, pp. 1413–1415.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © Yu.A. Stolpovsky, M. Ahani Azari, A.N. Evsukov, N.V. Kol, M.N. Ruzina, V.N. Voronkova, G.E. Sulimova, 2011, published in Genetika, 2011, Vol. 47, No. 2, pp. 213–226.
Rights and permissions
About this article
Cite this article
Stolpovsky, Y.A., Ahani Azari, M., Evsukov, A.N. et al. Comparison of ISSR polymorphism among cattle breeds. Russ J Genet 47, 189–200 (2011). https://doi.org/10.1134/S1022795410121051
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795410121051