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Exogenous Application of Jasmonic Acid Triggers the Rice Defense Mechanisms against Rhizoctonia solani Kühn

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Abstract

Rice sheath blight is caused by the necrotrophic soil-borne fungus Rhizoctonia solani Kühn. It is one of the most destructive rice disease. Jasmonic acid (JA) plays a vital role in plant defense mechanisms. This study aims to increase the understanding of JA in plant defense mechanisms against R. solani. In this study, we applied four different JA concentrations: 0.01, 0.1, 0.5, and 1 mM. JA application significantly increased the plant resistance against R. solani. The highest relative control of 82.38% was achieved by plants treated with a concentration of 0.1 mM. JA concentration of 0.1 mM is the most suitable treatment and above this concentration, the lesion length started to increase which indicates the reduction of plant resistance. Treatment 0.1 mM JA is further used to investigate defense-related enzymes and the expression of genes. 0.1 mM JA treatment effectively increases the activity of enzymes involved in plant defense mechanisms such as phenylalanine ammonia-lyase (PAL), peroxidase (POD), and β-1,3-glucanase, JA also lowers the malondialdehyde (MDA) content which proves its effectiveness against R. solani. The POX, PAL, and OsPR1b genes are the key marker genes for the plant defense, and the application of JA positively increases the relative expressions of these genes. These results confirm the significance of JA in plant defense mechanisms.

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ACKNOWLEDGMENTS

The authors would like to thank Northeast Agricultural University, Harbin, China for their support. The authors are grateful to Prof. Zhang Junhua for his support and continuous encouragement.

Funding

This research has been supported by Natura Fund Project, Heilongjiang, China, grant number C2017032, Major project of Applied Technology research and development program of Heilongjiang, grant Number GA19B104, and National Science and Technology project of grain high yield (2018YFD0300105-5).

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Authors and Affiliations

  1. College of Agriculture, Northeast Agricultural University, 150030, Harbin, China

    H. Younis, Z. Qingyan, L. L. Fu, P. Lili, Y. Songrun, M. Rexiti, Y. Shuo, S. Y. Wei, F. Siddique, W. Meng & Z. Junhua

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  1. H. Younis
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  2. Z. Qingyan
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  3. L. L. Fu
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  4. P. Lili
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  5. Y. Songrun
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  6. M. Rexiti
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  7. Y. Shuo
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  8. S. Y. Wei
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  9. F. Siddique
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  10. W. Meng
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  11. Z. Junhua
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Contributions

Haseeb Younis designed the experiments. Zhong Qingyan, Liu Lian Fu, Yang Songrun and Peng Lili collected samples and performed the experiments. Mukadasi Rexiti, Yang Shuo, Shu Yu Wei, Faisal Siddique and Wang Meng, drafted the manuscript. Zhang Junhua supervised the research and all authors revised it.

Corresponding author

Correspondence to Z. Junhua.

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COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants as the object of research.

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Additional information

Abbreviations: ET—ethylene; JA—jasmonic acid; MDA—malondialdehyde; MeJA—methyl jasmonate; PAL—phenylalanine ammonia lyase; POD—peroxidase; PPO—polyphenol oxidase; qRT‑PCR—quantitative real-time PCR; SA—salicylic acid.

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Younis, H., Qingyan, Z., Fu, L.L. et al. Exogenous Application of Jasmonic Acid Triggers the Rice Defense Mechanisms against Rhizoctonia solani Kühn. Russ J Plant Physiol 69, 167 (2022). https://doi.org/10.1134/S1021443722601665

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