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JMJD3 Exerts Oncorepressor Activity in Acute Promyelocytic Leukemia by Promoting PU.1 Expression

  • MOLECULAR BIOLOGY OF THE CELL
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Abstract

All-trans retinoic acid (ATRA) in acute promyelocytic leukemia (APL) has been the most famous differentiation induction therapy during which the expression of PU.1, a key transcription factor (TF) for myeloid lineage determination in normal hematopoiesis is restored. In our previous studies, we found a stress-inducible H3K27 demethylase, JMJD3, to directly upregulate PU.1 expression to promote myeloid commitment during normal myelopoiesis. In addition, JMJD3 acts as an oncorepressor and plays a critical regulatory role in the initiation and progression of malignant hematopoiesis. In this study, we further resolved the relationship between JMJD3 and PU.1 in APL therein JMJD3 exerts oncorepressor activity via promoting PU.1 expression.

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ADDITIONAL INFORMATION

The text was submitted by the author(s) in English.

The accession number for the raw data of RNA-seq and ChIP-seq reported in this paper is GEO: GSE101891. All other relevant data are available from the corresponding author on request.

Funding

This work was supported by the National Natural Science Foundation of China (NSFC) (81900148), and Shanghai Rising-Star Program (20QC1400100).

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Authors and Affiliations

  1. Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China

    Meng-Xi Wang, Shan-He Yu & Juan Chen

  2. Department of Hematology, Xuzhou Central Hospital, Xuzhou Medical University, 221009, Xuzhou, China

    Meng-Xi Wang

  3. Shanghai Ji Ai Genetics and IVF Institute, the Obstetrics and Gynecology Hospital of Fudan University, 200011, Shanghai, China

    Min Xiao

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  1. Meng-Xi Wang
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Contributions

MXW, SHY, MX, and JC conducted the study design, data acquisition, and analyses. MXW, SHY, MX, and JC carried out the experiments and drafted the manuscript.

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Correspondence to Min Xiao or Juan Chen.

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Conflicts of interest. The authors declare no conflicts of interest.

Statement of compliance with standards of research involving humans as subjects. The work has been carried out in accordance with The Code of Ethics of the World Medical Association (Declaration of Helsinki) and informed consent was obtained from the participant.

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Wang, MX., Yu, SH., Xiao, M. et al. JMJD3 Exerts Oncorepressor Activity in Acute Promyelocytic Leukemia by Promoting PU.1 Expression. Mol Biol 57, 653–660 (2023). https://doi.org/10.1134/S0026893323040179

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  • DOI: https://doi.org/10.1134/S0026893323040179

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