Abstract—
Homeodomain transcription factors play a significant role in adipocyte differentiation. The role of Pbx1 and Prep1, proteins of the TALE family (the three amino acid loop extension), was previously established in adipocyte differentiation of mesenchymal stromal cells and 3T3-L1 cell line. In this study, with the use of RNA interference technology we show that another transcription factor from the same family, Meis1, which is a core protein of mature cardiomyocytes, represses adipogenesis to a greater degree than its paralog Meis2. A number of Meis target genes, markers of adipocytes, are identified. This may indicate the transcriptional mechanism of the effect of Meis1 on the adipocyte differentiation of mouse preadipocytes.
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REFERENCES
Siersbæk R., Nielsen R., John S., Sung M.H., Baek S., Loft A., Hager G.L., Mandrup S. 2011. Extensive chromatin remodelling and establishment of transcription factor “hotspots” during early adipogenesis. EMBO J. 30, 1459–1472.
Siersbaek R., Nielsen R., Mandrup S. 2012. Transcriptional networks and chromatin remodeling controlling adipogenesis. Trends Endocrinol. Metab. 23, 56–64.
Fu M., Sun T., Bookout A.L., Downes M., Yu R.T., Evans R.M., Mangelsdorf D.J. 2005. A nuclear receptor atlas: 3T3-L1 adipogenesis. Mol. Endocrinol. 19, 2437–2450.
Siersbæk R., Baek S., Rabiee A., Nielsen R., Traynor S., Clark N., Sandelin A., Jensen O.N., Sung M.H., Hager G.L., Mandrup S. 2014. Molecular architecture of transcription factor hotspots in early adipogenesis. Cell Rep. 7, 1434–1442.
Monteiro M.C., Sanyal M., Cleary M.L., Sengenès C., Bouloumié A., Dani C., Billon N. 2011. PBX1: A novel stage-specific regulator of adipocyte development. Stem Cells. 29, 1837–1848.
Penkov D., Mateos San Martín D., Fernandez-Díaz L.C., Rosselló C.A., Torroja C., Sánchez-Cabo F., Warnatz H.J., Sultan M., Yaspo M.L., Gabrieli A., Tkachuk V., Brendolan A., Blasi F., Torres M. 2013. Analysis of the DNA-binding profile and function of TALE homeoproteins reveals their specialization and specific interactions with Hox genes/proteins. Cell Rep. 3, 1321–1333.
Maroni G., Tkachuk V.A., Egorov A., Morelli M.J., Luongo R., Levantini E., Blasi F., Magli M.C., Penkov D. 2017. Prep1 prevents premature adipogenesis of mesenchymal progenitors. Sci. Rep. 7, 15573.
Liu Q., Jiang C., Xu J., Zhao M.T., Van Bortle K., Cheng X., Wang G., Chang H.Y., Wu J.C., Snyder M.P. 2017. Genome-wide temporal profiling of transcriptome and open chromatin of early cardiomyocyte differentiation derived from hiPSCs and hESCs. Circ. Res. 121, 376–391.
Quaranta R., Fell J., Rühle F., Rao J., Piccini I., Araúzo-Bravo M.J., Verkerk A.O., Stoll M., Greber B. 2018. Revised roles of ISL1 in a hES cell-based model of human heart chamber specification. eLife. 7, e31706.
Moffat J., Grueneberg D.A., Yang X., Kim S.Y., Kloepfer A.M., Hinkle G., Piqani B., Eisenhaure T.M., Luo B., Grenier J.K., Carpenter A.E., Foo S.Y., Stewart S.A., Stockwell B.R., Hacohen N., et al. 2006. A lentiviral RNAi libraryfor human and mouse genes applied to an arrayed viral high-content screen. Cell. 124, 1283–1298.
Longobardi E., Blasi F. 2003. Overexpression of PREP-1 in F9 teratocarcinoma cells leads to a functionally relevant increase of PBX-2 by preventing its degradation. J. Biol. Chem. 278, 39235–39241.
Kraus N.A., Ehebauer F., Zapp B., Rudolphi B., Kraus B.J., Kraus D. 2016. Quantitative assessment of adipocyte differentiation in cell culture. Adipocyte. 5, 351–358.
McLean C.Y., Bristor D., Hiller M., Clarke S.L., Schaar B.T., Lowe C.B., Wenger A.M., Bejerano G. 2010. GREAT improves functional interpretation of cis-regulatory regions. Nat. Biotechnol. 28, 495–501.
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Translated by M. Novikova
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Greco, A., Vaipan, D.V., Tkachuk, V.A. et al. The Involvement of Cardiomyocyte-Specific Transcription Factors Meis in Adipocyte Differentiation. Mol Biol 53, 438–441 (2019). https://doi.org/10.1134/S0026893319030075
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DOI: https://doi.org/10.1134/S0026893319030075