This is a preview of subscription content, log in via an institution to check access.
REFERENCES
Babanazarova, O.V., Sidelev, S.I., and Fastner, J., Northern expansion of Cylindrospermopsis raciborskii (Nostocales, Cyanoprokaryota) observed in shallow highly eutrophic Lake Nero (Russia), Int. J. Algae, 2015, vol. 17, pp. 131–141.
Ballot, A., Fastner, J., and Wiedner, C., Paralytic shellfish poisoning toxin-producing cyanobacterium Aphanizomenon gracile in Northeast Germany, Appl. Environ. Microbiol., 2010, vol. 76, pp. 1173–1180.
Barón-Sola, Á., Ouahid, Y., and Campo, F., Detection of potentially producing cylindrospermopsin and microcystin strains in mixed populations of cyanobacteria by simultaneous amplification of cylindrospermopsin and microcystin gene regions, Ecotoxicol. Environ. Saf., 2012, vol. 75, pp. 102–108.
Hisbergues, M., Christiansen, G., Rouhiainen, L., Sivonen, K., and Börner, T., PCR-based identification of microcystin-producing genotypes of different cyanobacterial genera, Arch. Microbiol., 2003, vol. 180, pp. 402–410.
Molecular Tools for Detection and Quantification of Toxigenic Cyanobacteria, Kurmayer, R., Sivonen, K., Wilmotte, A., and Salmaso, N., Eds., Hoboken NJ: Wiley, 2017.
Rantala-Ylinen, A., Känä, S., Wang, H., Rouhiainen, L., Wahlsten, M., Rizzi, E., Berg, K., Gugger, M., and Sivonen, K., Anatoxin-a synthetase gene cluster of the cyanobacterium Anabaena sp. strain 37 and molecular methods to detect potential producers, Appl. Environ. Microbiol., 2011, vol. 77, pp. 7271–7278.
Semenova, A.S., Sidelev, S.I., and Dmitrieva, O.A., Experimental investigation of natural populations of Daphnia galeata G.O. Sars from the Curonian Lagoon feeding on potentially toxigenic cyanobacteria, Biol. Bull., 2017, vol. 44, pp. 538–546.
Sidelev, S.I., Golokolenova, T.B., Chernova, E.N., and Russkikh, Y.V., Analysis of phytoplankton in Tsimlyansk Reservoir (Russia) for the presence of cyanobacterial hepato- and neurotoxins, Microbiology (Moscow), 2015, vol. 84, pp. 828–837.
Sidelev, S.I., Korneva, L.G., Solovyeva, V.V., Zubishina, A.A., and Pligin, D.N., Molecular genetic identification and seasonal succession of toxigenic cyanobacteria in phytoplankton of the Rybinsk Reservoir (Russia), Inland Water Biol., 2016, vol. 9, pp. 368–374.
FUNDING
The work was supported by a grant from the President of the Russian Federation no. MK-1284.2013.5 , preparation of the communication was supported by the Russian Foundation for Basic Research, project no. 18-04-01069-а and by the project of the Yaroslavl State University no. OP-2G-08/2018.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Сonflict of interests. The authors declare that they have no conflict of interest.
Statement on the welfare of animals. This article does not contain any studies involving animals performed by any of the authors.
Additional information
Translated by P. Sigalevich
Rights and permissions
About this article
Cite this article
Sidelev, S.I. A Novel Multiplex PCR-based Technique for Detection of Toxigenic Cyanobacteria. Microbiology 88, 375–377 (2019). https://doi.org/10.1134/S0026261719030123
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0026261719030123