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Iron-Reducing Microbial Communities of the Lake Baikal Low-Temperature Bottom Sediments

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Abstract—

Psychroactive enrichment cultures reducing anthraquinone 2,6-disulfonate (AQDS) and soluble complexes of ferric iron at 5–20°C were isolated from the samples of Lake Baikal bottom sediments collected at the depths of 404 to 1396 m. Cultivation resulted in production of up to 6 mM Fe(II), which was over 50% of the initial Fe(III) concentration in the medium, and of 5.5 mM AH2QDS (~30% of the initial quinone concentration). The enrichment culture of iron-reducing bacteria St3 used Fe(III) citrate as the terminal electron acceptor, oxidizing formate from 6.5 to 2.0 g L–1 at 15°C. Phylogenetic analysis showed that the initial samples of the Lake Baikal bottom sediments and the enrichments obtained from these samples contained the taxa of classes Alpha- and Betaproteobacteria, which were closely related to bacteria capable of oxidizing aromatic compounds using inorganic electron acceptors, including ferric iron.

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ACKNOWLEDGMENTS

The authors are grateful to T.I. Zemskaya (Laboratory of Carbohydrate Microbiology, Limnological Institute, Siberian Branch, Russian Academy of Sciences) and to O.P. Dagurova (Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences) for providing Lake Baikal bottom sediments. The work was supported by the Russian Foundation for Basic Researh, projects nos. 12-04-31353 and 12-05-01085.

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  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia

    A. G. Zakharyuk, Ya. V. Ryzhmanova, A. N. Avtukh & V. A. Shcherbakova

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  1. A. G. Zakharyuk
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  2. Ya. V. Ryzhmanova
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  3. A. N. Avtukh
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  4. V. A. Shcherbakova
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Correspondence to A. G. Zakharyuk.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by P. Sigalevich

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Zakharyuk, A.G., Ryzhmanova, Y.V., Avtukh, A.N. et al. Iron-Reducing Microbial Communities of the Lake Baikal Low-Temperature Bottom Sediments. Microbiology 88, 156–163 (2019). https://doi.org/10.1134/S0026261719020139

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  • DOI: https://doi.org/10.1134/S0026261719020139

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