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Investigation of the Diffusion of Methylene Blue through Dentin from a Human Tooth

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Abstract

The diffusion of an aqueous solution of methylene blue through a section of dentin from a human tooth was studied using diffuse reflectance spectroscopy. A method based on a modified Bouguer–Lambert–Beer law was applied for the quantitative assessment of the rate of diffusion, and experimental data were fitted by the approximation within the free-diffusion model. For the first time, the coefficient of the diffusion of methylene blue through a section of dentin from a human tooth ex vivo was calculated. The mean value of the diffusion coefficient was (6.74 ± 1.32) × 10–6 cm2/s. The microelement composition of the samples was determined using the X-ray fluorescence method and scanning electron microscopy. The morphology of the surface of various parts of sections of the human tooth, for which the dye diffusion was investigated, was studied.

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ACKNOWLEDGMENTS

We are grateful to Yu.S. Skibina, director of the LLC Nanostructured Glass Technology, for her assistance in preparing samples, and A.M. Zakharevich, head of the Laboratory Diagnostics of Nanomaterials and Structures, Saratov National Research State University, for help in the study of surface morphology of samples of a human tooth by scanning electron microscopy. The work by V.V. Tuchin was supported by Russian Foundation for Basic Research (project nos. 17-00-00272 and 17-00-00275-K).

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Authors and Affiliations

  1. Chernyshevskii Saratov National Research State University, 410012, Saratov, Russia

    A. A. Selifonov & V. V. Tuchin

  2. Razumovskii Saratov State Medical University, 410012, Saratov, Russia

    A. A. Selifonov

  3. St. Petersburg National Research University of Information Technologies, Mechanics, and Optics, 197101, St. Petersburg, Russia

    V. V. Tuchin

  4. Institute of Precision Mechanics and Control, Russian Academy of Sciences, 410028, Saratov, Russia

    V. V. Tuchin

Authors
  1. A. A. Selifonov
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  2. V. V. Tuchin
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Correspondence to A. A. Selifonov.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

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Translated by M. Batrukova

Abbreviations: PS, photosensitizer; MB, methylene blue.

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Selifonov, A.A., Tuchin, V.V. Investigation of the Diffusion of Methylene Blue through Dentin from a Human Tooth. BIOPHYSICS 63, 981–988 (2018). https://doi.org/10.1134/S0006350918060222

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