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Acrylate Reductase of an Anaerobic Electron Transport Chain of the Marine Bacterium Shewanella woodyi

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Abstract

Many microorganisms are capable of anaerobic respiration in the absence of oxygen, by using different organic compounds as terminal acceptors in electron transport chain. We identify here an anaerobic respiratory chain protein responsible for acrylate reduction in the marine bacterium Shewanella woodyi. When the periplasmic proteins of S. woodyi were separated by ion exchange chromatography, acrylate reductase activity copurified with an ArdA protein (Swoo_0275). Heterologous expression of S. woodyi ardA gene (swoo_0275) in Shewanella oneidensis MR-1 cells did not result in the appearance in them of periplasmic acrylate reductase activity, but such activity was detected when the ardA gene was co-expressed with an ardB gene (swoo_0276). Together, these genes encode flavocytochrome c ArdAB, which is thus responsible for acrylate reduction in S. woodyi cells. ArdAB was highly specific for acrylate as substrate and reduced only methacrylate (at a 22-fold lower rate) among a series of other tested 2-enoates. In line with these findings, acrylate and methacrylate induced ardA gene expression in S. woodyi under anaerobic conditions, which was accompanied by the appearance of periplasmic acrylate reductase activity. ArdAB-linked acrylate reduction supports dimethylsulfoniopropionate-dependent anaerobic respiration in S. woodyi and, possibly, other marine bacteria.

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Abbreviations

Ard:

acrylate reductase

DMSO:

dimethyl sulfoxide

DMSP:

dimethylsulfoniopropionate

HPLC:

high performance liquid chromatography

MS:

mass spectrometry

MV:

methyl viologen

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Acknowledgments

MALDI MS facility became available to us in the framework of the Moscow State University Development Program PNG 5.13.

Funding

This work was supported by the Russian Science Foundation (project no. 24-24-00043).

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Authors and Affiliations

  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119234, Moscow, Russia

    Yulia V. Bertsova, Marina V. Serebryakova, Vladimir A. Bogachev, Alexander A. Baykov & Alexander V. Bogachev

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  1. Yulia V. Bertsova
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  2. Marina V. Serebryakova
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  3. Vladimir A. Bogachev
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  4. Alexander A. Baykov
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  5. Alexander V. Bogachev
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Contributions

A.V.B. the conception of the study; Y.V.B., M.V.S., V.A.B., A.A.B., and A.V.B. the acquisition and analysis of the data; A.A.B. and A.V.B. writing of the manuscript.

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Correspondence to Alexander V. Bogachev.

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Bertsova, Y.V., Serebryakova, M.V., Bogachev, V.A. et al. Acrylate Reductase of an Anaerobic Electron Transport Chain of the Marine Bacterium Shewanella woodyi. Biochemistry Moscow 89, 701–710 (2024). https://doi.org/10.1134/S0006297924040096

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