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Single Cell Proteogenomics — Immediate Prospects

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Abstract

Recent technical advances in genomic technology have led to the explosive growth of transcriptome-wide studies at the level of single cells. The review describes the first steps of the single cell proteomics that has originated soon after development of transcriptomics methods. The first studies on the shotgun proteomics of single cells that used liquid chromatography/mass spectrometry have been already published. In these works, the cells were separated by the methods used in transcriptomics studies (e.g., cell sorting) and analyzed by modified mass spectrometry with tandem mass tags. The new proteogenomics approach involving integration of single cell transcriptomics and proteomics data will provide better understanding of the mechanisms of cell interactions in normal development and disease.

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Abbreviations

ADAR:

adenosine deaminase

RNA:

dependent

FACS:

fluorescence-activated cell sorting

FISSEQ:

in situ fluorescence RNA sequencing

NGS:

next generation sequencing

SCoPE-MS:

Single Cell ProtEomics by Mass Spectrometry

TMT:

tandem mass tag

UMI:

unique molecular identifier

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Funding

The work was supported by the Russian Science Foundation (project 17–15–01229).

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Authors and Affiliations

  1. Pirogov Russian National Research Medical University, 117997, Moscow, Russia

    S. A. Moshkovskii

  2. Orekhovich Institute of Biomedical Chemistry, 119121, Moscow, Russia

    S. A. Moshkovskii

  3. Talrose Institute for Energy Problems of Chemical Physics, Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences, 119334, Moscow, Russia

    A. A. Lobas & M. V. Gorshkov

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  1. S. A. Moshkovskii
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  2. A. A. Lobas
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Correspondence to S. A. Moshkovskii.

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Conflict of interest. The authors declare no conflict of interest in financial of any other sphere.

Published in Russian in Biokhimiya, 2020, Vol. 85, No. 2, pp. 165-173.

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Moshkovskii, S.A., Lobas, A.A. & Gorshkov, M.V. Single Cell Proteogenomics — Immediate Prospects. Biochemistry Moscow 85, 140–146 (2020). https://doi.org/10.1134/S0006297920020029

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