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Apoptosis in cryopreserved eukaryotic cells

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Abstract

This review considers apoptosis mechanisms that have been revealed in cryopreserved cells and which can be controlled using different chemical agents, thereby improving the viability of cells after their return to normal conditions. The role of oxidative stress as of the most significant damaging factor is discussed, as well as the reasonability of including antioxidants into cryopreservation/thawing protocols as independent agents or in combination with other compounds.

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Abbreviations

DMSO:

dimethyl sulfoxide

PARP:

poly(ADP-ribose) polymerase

PS:

phosphatidylserine

ROCK:

Rho-associated protein kinase

ROS:

reactive oxygen species

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  1. Lomonosov Moscow State University, 119991, Moscow, Russia

    M. A. Savitskaya & G. E. Onishchenko

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  1. M. A. Savitskaya
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Original Russian Text © M. A. Savitskaya, G. E. Onishchenko, 2016, published in Biokhimiya, 2016, Vol. 81, No. 5, pp. 602-611.

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Savitskaya, M.A., Onishchenko, G.E. Apoptosis in cryopreserved eukaryotic cells. Biochemistry Moscow 81, 445–452 (2016). https://doi.org/10.1134/S0006297916050023

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  • DOI: https://doi.org/10.1134/S0006297916050023

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